Isolation and characterization of racemase from Ensifer sp. 23-3 that acts on α-aminolactams and α-amino acid amides
Isolation and characterization of racemase from Ensifer sp. 23-3 that acts on α-aminolactams and α-amino acid amides
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作用于 α-氨基内酰胺和 α-氨基酸酰胺的 Ensifer sp. 23-3 消旋酶的分离和表征
DOI：
10.1007/s10295-017-1981-5
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发表时间：
2017
影响因子：
3.4
通讯作者：
Asano Yasuhisa
中科院分区：
文献类型：
--
作者：
Matsui Daisuke;Fuhshuku Ken-ichi;Nagamori Shingo;Takata Momoko;Asano Yasuhisa
关于α-氨基-ε-己内酰胺（ACL）消旋酶（ACLR）的信息有限，ACLR是一种作用于ACL和α-氨基酸酰胺的吡哆醛5′-磷酸依赖性酶。本研究分离到8株对α-氨基-ε-己内酰胺具有外消旋能力的细菌，其中1株经鉴定为Ensifersp.菌株23-3。从Ensifersp. 23-3在大肠杆菌中克隆表达。然后从E.大肠杆菌的ACLR基因。23-3，并对其性质进行了表征。该酶不仅作用于ACL，还作用于α-氨基-δ-戊内酰胺、α-氨基-ω-辛内酰胺、α-氨基丁酸酰胺和丙氨酸酰胺。
Limited information is available on α-amino-ε-caprolactam (ACL) racemase (ACLR), a pyridoxal 5′-phosphate-dependent enzyme that acts on ACL and α-amino acid amides. In the present study, eight bacterial strains with the ability to racemize α-amino-ε-caprolactam were isolated and one of them was identified asEnsifersp. strain 23-3. The gene for ACLR fromEnsifersp. 23-3 was cloned and expressed inEscherichia coli. The recombinant ACLR was then purified to homogeneity from theE. colitransformant harboring the ACLR gene fromEnsifersp. 23-3, and its properties were characterized. This enzyme acted not only on ACL but also on α-amino-δ-valerolactam, α-amino-ω-octalactam, α-aminobutyric acid amide, and alanine amide.