Heat Shock of Porcine Zygotes Immediately After Oocyte Activation Increases Viability
Heat Shock of Porcine Zygotes Immediately After Oocyte Activation Increases Viability
复制标题
DOI：
10.1002/mrd.20975
复制
发表时间：
2009-06-01
影响因子：
2.5
通讯作者：
Rucker, Edmund B., III
中科院分区：
文献类型：
--
作者：
Isom, S. Clay;Lai, Liangxue;Rucker, Edmund B., III
我们评估了不同时间变化的应用热休克对孤雌激活（PA）猪胚胎的影响。PA胚胎在42 ℃下从活化后0-9小时（hpa; 09 HS）、13-22 hpa（1322 HS）或22-31 hpa（2231 HS）热休克9小时。对24小时卵裂率（P < 0.0001）、第5天细胞数（P < 0.005）、第7天囊胚率（P < 0.0001）和第7天细胞数（P < 0.05）的分析表明，09 HS胚胎在体外发育比所有其他处理和对照胚胎更成功。将体外受精（IVF）胚胎暴露于与PA胚胎所述相似的热处理，将体细胞核移植（SCNT）衍生的胚胎仅暴露于对照和09 HS处理，以评估不同热处理对首次卵裂和发育至胚泡时间的影响。与NHS对照组相比，IVF和SCNT来源的胚胎在受精或融合/激活后立即热休克时在发育第1天显示出更高比例的卵裂胚胎（P < 0.05）。然而，与对照胚胎相比，囊胚率仅显示出适度（IVF; P = 0.089）或无（SCNT; P > 0.1）改善。总之，卵母细胞激活后立即将PA胚胎暴露于高温导致发育潜力显著增强。对这种现象的彻底表征可能会产生一些发现，这些发现可以提高体外生产PA，IVF和SCNT胚胎的效率。
We evaluated the effect of different timing variations of an applied heat shock on parthenogenetically activated (PA) porcine embryos. PA embryos were heat shocked for 9 hr at 42 degrees C from either 0-9 hr post activation (hpa; 09HS), 13-22 hpa (1322HS), or 22-31 hpa (2231HS). Analysis of 24-hr cleavage rates (P < 0.0001), day 5 cell numbers (P < 0.005), day 7 blastocyst rates (P < 0.0001), and day 7 cell numbers (P < 0.05) showed that 09HS embryos developed more successfully in vitro than did all other treated and control embryos. In vitro fertilized (IVF) embryos were exposed to similar heat treatments as described for PA embryos, and embryos derived from somatic cell nuclear transfer (SCNT) were exposed only to the control and 09HS treatments to assess the effects of the different heat treatments on the timing of first cleavage and development to blastocyst. Embryos derived from both IVF and SCNT showed higher proportions of cleaved embryos on day 1 of development when heat shocked immediately after fertilization or fusion/activation as compared to NHS controls (P < 0.05). Blastocyst rates however, showed only modest (IVF; P = 0.089) or no (SCNT; P > 0.1) improvement as compared with control embryos. In summary, exposing PA embryos to elevated temperatures immediately after oocyte activation results in dramatically enhanced developmental potential. A thorough characterization of this phenomenon may yield findings that can serve to increase the efficiency with which PA, IVF, and SCNT embryos are produced in vitro.