Establishment and characterization of a human malignant fibrous histiocytoma cell line.
Establishment and characterization of a human malignant fibrous histiocytoma cell line.
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人恶性纤维组织细胞瘤细胞系的建立和表征。
DOI：
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发表时间：
1995
影响因子：
4.2
通讯作者：
H. Moriya
中科院分区：
文献类型：
--
作者：
T. Yonemoto;T. Takenouchi;H. Tokita;S. Tatezaki;N. Mukaida;A. Mikata;H. Moriya
恶性纤维组织细胞瘤是最常见的软组织肉瘤。然而，发病机制仍然不清楚，因为有很少的人恶性纤维组织细胞瘤细胞系可用于精确的细胞研究。在这项研究中，一个人恶性纤维组织细胞瘤细胞系（MMF-1）建立从肺转移病灶的55岁男子恶性纤维组织细胞瘤。人细胞系MMF-1及其异种移植瘤在形态学和免疫化学上与原瘤基本相同。该细胞系有望成为研究恶性纤维组织细胞瘤发病机制的有用细胞。克隆的细胞系（MMF-2和MMF-3）也由梭形、多边形和多核巨细胞组成，这意味着恶性纤维组织细胞瘤中观察到的成纤维细胞样细胞、组织细胞样细胞和多核巨细胞来源于单个肿瘤细胞。人细胞系MMF-1产生的炎性细胞因子，如肿瘤坏死因子-α，巨噬细胞集落刺激因子，白细胞介素-8，单核细胞趋化和活化因子，可能参与恶性纤维组织细胞瘤的形态发生。此外，人细胞系MMF-1的分析结果表明，恶性纤维组织细胞瘤起源于低分化的成纤维细胞。
Malignant fibrous histiocytoma is the most frequent soft tissue sarcoma. However, the pathogenesis still remains unclear, because there are very few human malignant fibrous histiocytoma cell lines available for precise cellular study. In this study, a human malignant fibrous histiocytoma cell line (MMF-1) was established from the pulmonary metastatic lesion of a 55-year-old man with malignant fibrous histiocytoma. Human cell line MMF-1 and its heterotransplanted tumor had almost the same characteristics as the original tumor morphologically and immunohistochemically. This cell line is expected to be a useful for studying the pathogenesis of malignant fibrous histiocytoma. The cloned cell lines (MMF-2 and MMF-3) also consisted of spindle-shaped, polygonal, and multinucleated giant cells, meaning that the fibroblast-like cells, histiocyte-like cells, and multinucleated giant cells seen in malignant fibrous histiocytoma were derived from a single tumor cell. Human cell line MMF-1 produced inflammatory cytokines, such as tumor necrosis factor-alpha, macrophage colony-stimulating factor, interleukin-8, and monocyte chemotactic and activating factor, that might be involved in the morphogenesis of malignant fibrous histiocytoma. Furthermore, the results of the analysis of human cell line MMF-1 suggested that malignant fibrous histiocytoma originated from a poorly differentiated fibroblast.
酶免疫组织化学：技术方面。
影响因子：
2.3
作者：
Sheibani,K;Tubbs,RR
通讯作者：
Tubbs,RR