Effect of low-level basal plus marked ''pulsatile'' hyperglycemia on insulin secretion in fetal sheep
Effect of low-level basal plus marked ''pulsatile'' hyperglycemia on insulin secretion in fetal sheep
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DOI：
10.1152/ajpendo.1996.271.5.e865
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发表时间：
1996-11-01
影响因子：
5.1
通讯作者：
Hay, WW
中科院分区：
文献类型：
--
作者：
Carver, TD;Anderson, SM;Hay, WW
我们比较了四组妊娠绵羊在不同母体血糖模式下10-11天后的胎儿葡萄糖和精氨酸刺激的胰岛素分泌（Delta I，pM）：1）对照，正常血糖; 2）低水平基础加“脉冲”高血糖（PHG组）; 3）显著高血糖（HG组）; 4）显著低血糖（LG组）。与对照组（126 +/- 18 pM）相比，高血糖钳夹期间PHG组（190 +/- 28 pM，P < 0.01）的平均Delta I最大，HG组（64 +/- 13 pM，P <0.05）和LG组（69 +/- 15 pM，P < 0.05）最小。精氨酸推注后，PHG组在30分钟内四个采样时间中的两个采样时间的胰岛素浓度高于对照组，并且在所有时间均高于HG和LG组。在麦角碱后采样期间（PHG 1，092 +/-114 pM;对照921 +/-86 pM; HG 897 +/-117 pM; LG 831 +/-57 pM）的平均Delta I的趋势与葡萄糖的趋势相同，并且是显著的（P < 0.05）。因此，葡萄糖刺激的胎儿胰岛素分泌受母体和胎儿高血糖的持续时间和模式以及幅度的调节;这种调节也可能延伸到胰岛素分泌能力。
We compared fetal glucose- and arginine-stimulated insulin secretion (Delta I, pM) among four groups of pregnant sheep after 10-11 days of different maternal glycemic patterns: 1) control, euglycemic; 2) low-level basal plus ''pulsatile'' hyperglycemic (PHG group); 3) markedly hyperglycemic (HG group); 4) markedly hypoglycemic (LG group). Mean Delta I during a hyperglycemic clamp was greatest in the PHG group (190 +/- 28 pM, P < 0.01) and least in the HG (64 +/- 13 pM, P < 0.05) and LG groups (69 +/- 15 pM, P < 0.05) compared with the control group (126 +/- 18 pM). After an arginine bolus, insulin concentration was greater in the PHG group at two of four sampling times over 30 min compared with the control group and at all times compared with the HG and LG groups. The trend in mean Delta I over the postarginine sampling period (PHG 1,092 +/- 114 pM; control 921 +/- 86 pM; HG897 +/- 117 pM; LG831 +/- 57 pM) was in the same direction as for glucose and was significant (P < 0.05). Thus glucose-stimulated fetal insulin secretion is regulated by the duration and pattern, as well as the magnitude, of maternal and fetal hyperglycemia; this regulation may also extend to insulin-secretion capacity.