Effect of Tiletamine on the Activity of NOS,NO Production,and cGMP Content in Rat Different Brain Regions
Effect of Tiletamine on the Activity of NOS,NO Production,and cGMP Content in Rat Different Brain Regions
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发表时间：
2008
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通讯作者：
Zhang Jian-tao
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作者：
Zhang Jian-tao
通过观察替乐胺对大鼠不同脑区NOS活性、NO生成及cGMP含量的影响，探讨替乐胺介导全身麻醉中NO/cGMP信号转导的分子机制。168SD大鼠随机分为对照组、高剂量替乐胺组（腹腔注射替乐胺60 mg/kg）和低剂量替乐胺组（腹腔注射替乐胺30 mg/kg），每个剂量组又分为3个亚组，如麻醉组、恢复组Ⅰ和恢复组Ⅱ。分光光度法测定大鼠不同脑区NOS活性和NO生成量，放射免疫法测定大鼠不同脑区cGMP含量。给药后，麻醉组大鼠大脑皮层、海马和丘脑NOS活性明显受到抑制，上述区域NO生成和cGMP含量明显降低（与对照组比较，P0.05）。两种剂量下，恢复组NOS活性、NO生成量和cGMP含量Ⅰ均有不同程度恢复，除恢复组丘脑cGMP含量Ⅱ与对照组相比，均有显著恢复（与对照组相比，P0.05）。不同剂量替乐他明对大鼠脑干和小脑NOS活性、NO生成及cGMP含量均有显著影响。替乐胺的麻醉作用可能与抑制大脑皮质、丘脑和海马的NO/cGMP信号转导有关。
The effect of tiletamine on the activity of NOS,NO production,and cGMP content in different brain regions of rat were observed to investigate the modulation of NO/cGMP signal transduction in the molecular mechanisms of general anaesthesia mediated by tiletamine.168 SD rats were randomly divided into conrol group,high dose tiletamine group(intraperitoneally tilelamine 60 mg/kg) and low dose tiletamine group(ip tilelamine 30 mg/kg),and each dose group was divided into three sub-groups,eg anesthesia group,recovery groupⅠand recovery groupⅡ.The activity of NOS and production of NO were measured by spectrophotometric analysis,and the cGMP content were measured by radioimmunoassay(RIA) in different brain regions.After administering tiletamine,the activity of NOS in the cerebral cortex,the hippocampus and the thalamus in the anesthesia group were obviously inhibited,and the NO production and cGMP content in above-mentioned regions were obviously decreased(compared with control group,P0.05).The activity of NOS,NO production and cGMP content in recovery groupⅠat the two dosage were recoverd in different degree,and they were significant recovered(compared with control group,P0.05)other than cGMP content in the thalamus(compared with control group,P0.05) in the recovery groupⅡ.Different dose of tiletamine affected significantly the activity of NOS,NO production and cGMP content in the brain stem,and cerebellum.The anesthesia effects of tiletamine might relate to inhibition of NO/cGMP signal transduction in cerebral cortex,the thalamus,and the hippocampus.