Molecular recognition of small-cell lung cancer cells using aptamers
Molecular recognition of small-cell lung cancer cells using aptamers
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DOI：
10.1002/cmdc.200800030
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发表时间：
2008-06-01
期刊：
影响因子：
3.4
通讯作者：
Tan, Weihong
中科院分区：
文献类型：
--
作者：
Chen, Hui William;Medley, Colin D.;Tan, Weihong
早期诊断是提高肺癌生存率的方法，但由于缺乏敏感和选择性识别肺癌细胞的分子探针，目前几乎不可能。我们开发了一种新的适体方法，用于识别特定的小细胞肺癌（SCLC）细胞表面分子标记。我们的方法依赖于基于细胞的配体系统进化，通过指数富集（cell-SELEX）来进化表达各种表面标志物的全活细胞的适配体，这些表面标志物代表癌细胞之间的分子差异。当应用于不同的肺癌细胞（包括来自患者样品的肺癌细胞）时，这些适体在各种测定形式中以高亲和力和特异性结合SCLC细胞。当与磁性和荧光纳米颗粒缀合时，适体纳米缀合物可以有效地从混合细胞培养基中提取SCLC细胞用于分离、富集和灵敏检测。这些研究证明了适体方法用于早期肺癌检测的潜力。
Early diagnosis is the way to improve the rate of lung cancer survival, but is almost impossible today due to the lack of molecular probes that recognize lung cancer cells sensitively and selectively. We developed a new aptamer approach for the recognition of specific small-cell lung cancer (SCLC) cell-surface molecular markers. Our approach relies on cell-based systematic evolution of ligands by exponential enrichment (cell-SELEX) to evolve aptamers for whole live cells that express a variety of surface markers representing molecular differences among cancer cells. When applied to different lung cancer cells including those from patient samples, these aptamers bind to SCLC cells with high affinity and specificity in various assay formats. When conjugated with magnetic and fluorescent nanoparticles, the aptamer nanoconjugates could effectively extract SCLC cells from mixed cell media for isolation, enrichment, and sensitive detection. These studies demonstrate the potential of the aptamer approach for early lung cancer detection.