A hybrid microfluidic-vacuum device for direct interfacing with conventional cell culture methods.
A hybrid microfluidic-vacuum device for direct interfacing with conventional cell culture methods.
复制标题
用于直接与常规细胞培养方法直接接口的杂化微流体-Vacuum设备。
DOI：
10.1186/1472-6750-7-60
复制
发表时间：
2007-09-20
影响因子：
3.5
通讯作者：
Jeon, Noo Li
中科院分区：
文献类型：
--
作者：
Chung, Bong Geun;Park, Jeong Won;Hu, Jia Sheng;Huang, Carlos;Monuki, Edwin S;Jeon, Noo Li
当需要精确控制细胞微环境时，微流体技术是一种比传统组织培养方法具有许多优点的技术。然而，有一些实际和技术上的限制阻碍了在常规生物医学研究中更广泛的应用。制造和设置微流体实验所需的专用设备和协议对大多数生物实验室的常规使用存在障碍。我们已经开发并验证了一种新型的微流体装置，它可以直接与传统的组织培养方法相结合，在培养皿中产生和维持可控的可溶性环境。它在单个设备上集成了单独的流体通道和真空网络，允许在湿细胞培养表面上可逆地应用微流体梯度。通过连接在灌注系统上的简单微流控通道，可以产生稳定、精确的可溶性因子浓度梯度。我们成功地展示了暴露于过氧化氢梯度下的神经干细胞的实时光学活/死细胞成像和转移性乳腺癌细胞在生长因子梯度下的趋化性。本文介绍了一种多功能微流体装置的设计和应用，该装置可以直接与传统的细胞培养方法相结合。该平台提供了一个简单而通用的工具，将微流体方法的优点纳入生物测定，而无需改变已建立的组织培养方案。
Microfluidics is an enabling technology with a number of advantages over traditional tissue culture methods when precise control of cellular microenvironment is required. However, there are a number of practical and technical limitations that impede wider implementation in routine biomedical research. Specialized equipment and protocols required for fabrication and setting up microfluidic experiments present hurdles for routine use by most biology laboratories. We have developed and validated a novel microfluidic device that can directly interface with conventional tissue culture methods to generate and maintain controlled soluble environments in a Petri dish. It incorporates separate sets of fluidic channels and vacuum networks on a single device that allows reversible application of microfluidic gradients onto wet cell culture surfaces. Stable, precise concentration gradients of soluble factors were generated using simple microfluidic channels that were attached to a perfusion system. We successfully demonstrated real-time optical live/dead cell imaging of neural stem cells exposed to a hydrogen peroxide gradient and chemotaxis of metastatic breast cancer cells in a growth factor gradient. This paper describes the design and application of a versatile microfluidic device that can directly interface with conventional cell culture methods. This platform provides a simple yet versatile tool for incorporating the advantages of a microfluidic approach to biological assays without changing established tissue culture protocols.