Expression of the dopamine transporter in the brain of the honeybee, Apis mellifera L. (Hymenoptera: Apidae)
Expression of the dopamine transporter in the brain of the honeybee, Apis mellifera L. (Hymenoptera: Apidae)
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DOI：
10.1303/aez.2009.403
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发表时间：
2009-08-01
影响因子：
1.3
通讯作者：
Sasaki, Masami
中科院分区：
文献类型：
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作者：
Nomura, Shogo;Takahashi, Jun-ichi;Sasaki, Masami
多巴胺转运蛋白（dopamine transporter， DAT）位于多巴胺能末端的突触前膜上，负责恢复突触间隙释放的多巴胺。因此，其表达水平有望反映多巴胺能神经元的活性。我们鉴定了数据（Amdat）的蜜蜂同源物，并通过逆转录实时定量聚合酶链反应测定了其在蜜蜂脑中的表达水平。工蜂以一种年龄依赖的方式将其任务从护理转变为觅食，并且发现它们大脑中的Amdat转录水平随着年龄的增长而增加。此外，早熟觅食者的表达水平显著高于同龄护士，表明Amdat的表达与任务转换有关。在蜂王中，Amdat的表达水平在交配飞行后下降。在雄蜂中，Amdat的表达随着年龄的增长而增加。我们的数据表明，多巴胺通路的活性与蜜蜂的行为活动有关。
The dopamine transporter (DAT) is located on the presynaptic membrane of dopaminergic terminals and recovers the released dopamine from synaptic clefts. Therefore, its expression level is expected to reflect the activity of dopaminergic neurons. We identified the honeybee ortholog of dat (Amdat) and measured its expression levels in honeybee brain by reverse transcription real-time quantitative polymerase chain reaction. Worker honeybees change their tasks from nursing to foraging in an age-dependent manner, and Amdat transcript levels in their brains were found to increase with age. Furthermore, the expression level was significantly higher in precocious foragers than in nurses of the same age, suggesting that Amdat expression is related to task shifts. In queens, Amdat expression levels decreased after a mating flight. Amdat expression in drone honeybees increased with age. Our data suggest that dopamine pathway activity is correlated with the behavioral activity of honeybee.