A midgut-specific chymotrypsin cDNA (Slctlp1) from Spodoptera litura: cloning, characterization, localization and expression analysis.
A midgut-specific chymotrypsin cDNA (Slctlp1) from Spodoptera litura: cloning, characterization, localization and expression analysis.
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DOI：
10.1002/arch.20353
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发表时间：
2011-03
影响因子：
2.2
通讯作者：
Qinglei Zhan;Sichun Zheng;Q. Feng;Lin Liu
中科院分区：
文献类型：
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作者：
Qinglei Zhan;Sichun Zheng;Q. Feng;Lin Liu
丝氨酸蛋白酶在昆虫发育过程中的食物消化和免疫应答中起着重要作用。从斜纹夜蛾（Spodoptera litura）中克隆了一个编码糜蛋白酶样丝氨酸蛋白酶的全长cDNA（Slctlp1），并对其cDNA结构、发育和诱导表达及定位进行了研究。Slctlp1 cDNA的推导的蛋白质包含在大多数丝氨酸蛋白酶中发现的催化三联体和底物特异性口袋。Slctlp1的转录本和蛋白质在幼虫发育过程中的摄食阶段主要在中肠表达。免疫组织化学分析表明，SLCTLP 1蛋白主要存在于6龄幼虫的中肠中。饥饿抑制了6龄幼虫Slctlp1基因和蛋白的表达，重新取食后，Slctlp1基因和蛋白的表达量再次增加。结果表明，克隆的Slctlp1 cDNA可能参与食物蛋白质的消化在摄食阶段的幼虫发育。
Serine proteases play important roles in food digestion and immune response during insect development. A full-length cDNA (Slctlp1) encoding a chymotrypsin-like serine protease was cloned from Spodoptera litura and characterized for its cDNA structure, developmental and induced expression and localization. The deduced protein of the Slctlp1 cDNA contains a catalytic triad and a substrate specificity pocket found in most of the serine proteases. Both the transcripts and protein of Slctlp1 were predominately expressed in the midgut at the feeding stages during the larval development. Immunohistochemistry analysis indicated that the SLCTLP1 protein was predominately present in the midgut of the 6th instar feeding larvae. Starvation suppressed the expression of Slctlp1 gene and protein in 6th instar larvae and the protein expression was increased again by re-feeding the insect. The results suggest that the cloned Slctlp1 cDNA may be involved in food protein digestion at the feeding stages during larval development.