Hexameric ring structure of human MCM10 DNA replication factor
Hexameric ring structure of human MCM10 DNA replication factor
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DOI：
10.1038/sj.embor.7401064
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发表时间：
2007-10-01
期刊：
影响因子：
7.7
通讯作者：
Williams, Gareth H.
中科院分区：
文献类型：
--
作者：
Okorokov, Andrei L.;Waugh, Alastair;Williams, Gareth H.
DNA复制因子小染色体维持10 （MCM10）是一种保守的、丰富的核蛋白，对起始激活至关重要。在复制前复合物向起始前复合物过渡的过程中，需要MCM10招募到复制起点，从而在MCM2- 7复合体DNA解旋酶和DNA聚合酶之间提供物理连接。在这里，我们报告了通过电子显微镜和单粒子分析确定的人类MCM10的分子结构。MCM10分子是一个环形六聚体，具有较大的中央通道和较小的侧通道以及内腔系统。这种结构以及生化数据表明，这种重要的蛋白质利用其结构为真核DNA复制所需的分子机制组装提供了一个对接模块。
The DNA replication factor minichromosome maintenance 10 ( MCM10) is a conserved, abundant nuclear protein crucial for origin firing. During the transition from pre-replicative complexes to pre-initiation complexes, MCM10 recruitment to replication origins is required to provide a physical link between the MCM2- 7 complex DNA helicase and DNA polymerases. Here, we report the molecular structure of human MCM10 as determined by electron microscopy and single-particle analysis. The MCM10 molecule is a ring-shaped hexamer with large central and smaller lateral channels and a system of inner chambers. This structure, together with biochemical data, suggests that this important protein uses its architecture to provide a docking module for assembly of the molecular machinery required for eukaryotic DNA replication.