Is oblique scanning laser ophthalmoscope applicable to human ocular optics? A feasibility study using an eye model for volumetric imaging.
Is oblique scanning laser ophthalmoscope applicable to human ocular optics? A feasibility study using an eye model for volumetric imaging.
复制标题
斜扫描激光检眼镜适用于人眼光学吗？
DOI：
10.1002/jbio.201960174
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发表时间：
2020
影响因子：
2.8
通讯作者：
Yi,Ji
中科院分区：
文献类型：
--
作者：
Shao,Wenjun;Song,Weiye;Yi,Ji
倾斜扫描激光检眼镜 (oSLO) 是一种新颖的成像方式，无需在大视场 (FOV) 上进行深度切片即可提供体积视网膜成像。它已在啮齿类动物眼中成功地进行了体积荧光素血管造影 (vFA) 的体内验证。然而，由于人眼光学器件的数值孔径 (NA) 较低，用于人眼视网膜成像的 oSLO 工程具有挑战性。为了克服这一挑战，我们实施光学设计以（a）在Scheimpflug条件下增加中间图像的角度，以及（b）使用柱面透镜扩大深度维度的放大倍数以实现足够的采样密度。此外，我们采用扫描和反扫描策略，形成紧凑的 oSLO 系统。我们的实验表明，当前的装置可以实现约 3 × 6 × 0.8mm3 的视场，横向和轴向分辨率分别为 7 和 41μm。这项可行性研究为未来体内人类视网膜成像迈出了重要的一步。
Oblique scanning laser ophthalmoscopy (oSLO) is a novel imaging modality to provide volumetric retinal imaging without depth sectioning over a large field of view (FOV). It has been successfully demonstrated in vivo in rodent eyes for volumetric fluorescein angiography (vFA). However, engineering oSLO for human retinal imaging is challenging because of the low numerical aperture (NA) of human ocular optics. To overcome this challenge, we implement optical designs to (a) increase the angle of the intermediate image under Scheimpflug condition, and (b) expand the magnification in the depth dimension with cylindrical lens to enable sufficient sampling density. In addition, we adopt a scanning‐and‐descaning strategy, resulting in a compact oSLO system. We experimentally show that the current setup can achieve a FOV of ~3 × 6 × 0.8 mm3, and the transverse and axial resolutions of 7 and 41 μm, respectively. This feasibility study serves an important step for future in vivo human retinal imaging.