Therapeutic benefit of urocortin in rats with intracerebral hemorrhage Laboratory investigation
Therapeutic benefit of urocortin in rats with intracerebral hemorrhage Laboratory investigation
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DOI：
10.3171/2011.8.jns101637
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发表时间：
2012-01-01
影响因子：
4.1
通讯作者：
Pang, Cheng-Yoong
中科院分区：
文献类型：
--
作者：
Liew, Hock-Kean;Hsu, Chih-Wei;Pang, Cheng-Yoong
Object.脑出血（ICH）约占所有中风死亡的15%。它经常引起脑水肿，导致脑体积扩大，对ICH结局产生负面影响。ICH诱导的脑水肿涉及炎症机制。作者的体外研究表明，尿皮质素（UCN）具有神经保护作用。因此，本研究旨在探讨UCN对脑出血大鼠的神经保护作用。将VII-S型胶原酶或自体血注入麻醉大鼠的单侧纹状体内诱导脑出血。在诱导ICH后1小时，将UCN（0.05、0.5和5 mg）注入同侧脑室。作者检查了损伤面积、脑含水量、血脑屏障通透性和神经功能。在同侧侧脑室注射UCN，能够穿透到受损的纹状体。UCN后处理可减轻ICH大鼠脑损伤面积、脑水肿、血脑屏障通透性，改善神经功能缺损。UCN后处理可剂量依赖性地改善脑出血大鼠的神经功能缺损，为脑出血或其他脑损伤患者提供了一种潜在的治疗药物。(DOI：10.3171/2011.8.JNS101637）
Object. Intracerebral hemorrhage (ICH) accounts for about 15% of all deaths due to stroke. It frequently causes brain edema, leading to an expansion of brain volume that exerts a negative impact on ICH outcomes. The ICH-induced brain edema involves inflammatory mechanisms. The authors' in vitro study shows that urocortin (UCN) exhibits antiinflammatory and neuroprotective effects. Therefore, the neuroprotective effect of UCN on ICH in rats was investigated.Methods. Intracerebral hemorrhage was induced by an infusion of bacteria collagenase type VII-S or autologous blood into the unilateral striatum of anesthetized rats. At 1 hour after the induction of ICH, UCN (0.05, 0.5, and 5 mg) was infused into the lateral ventricle on the ipsilateral side. The authors examined the injury area, brain water content, blood-brain barrier permeability, and neurological function.Results. The UCN, administered in the ipsilateral lateral ventricle, was able to penetrate into the injured striatum. Posttreatment with UCN reduced the injury area, brain edema, and blood-brain barrier permeability and improved neurological deficits of rats with ICH.Conclusions. Posttreatment with UCN through improving neurological deficits of rats with ICH dose dependently provided a potential therapeutic agent for patients with ICH or other brain injuries. (DOI: 10.3171/2011.8.JNS101637)