Evidence for erythrocyte lipid peroxidation in acute falciparum malaria
Evidence for erythrocyte lipid peroxidation in acute falciparum malaria
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DOI：
10.1016/s0035-9203(99)90180-3
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发表时间：
1999-01-01
影响因子：
2.2
通讯作者：
Nanda, NK
中科院分区：
文献类型：
--
作者：
Das, BS;Nanda, NK
为了评估急性恶性疟原虫疟疾患者红细胞氧化应激的程度，对102例恶性疟原虫疟疾患者和50例对照者的红细胞硫代巴比妥酸反应物质（ETBAR）以及细胞内、膜和细胞外抗氧化剂进行了测定。患者体内ETBAR的平均浓度显著高于对照组（P < 0.001），许多抗氧化剂的浓度显著低于对照组。在红细胞抗氧化剂中，患者的过氧化氢酶、还原型谷胱甘肽（GSH）和生育酚显著降低（分别为P < 0.05、0.001、0.001）。红细胞超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-Px）未降低至统计学显著水平。同样，血浆抗氧化剂抗坏血酸和白蛋白显著降低（P < 0.001），但尿酸盐没有降低。ETBAR与红细胞GSH、生育酚、血浆抗坏血酸、白蛋白呈负相关（P <0.001），与红细胞抗氧化酶活性无相关性。然而，在多元回归分析中，只有生育酚与ETBAR密切相关，其次是GSH和血浆抗坏血酸。ETBAR还与溶血指数如血红蛋白、血浆未结合胆红素和结合珠蛋白浓度相关性良好（均P < 0.001）。在2周后的随访中，ETBAR和不同的抗氧化剂达到接近对照水平。这些观察结果表明，在急性恶性疟疾红细胞的氧化应激增强，可能大大有助于溶血和贫血。
To assess the extent of oxidative stress in erythrocytes of patients with acute Plasmodium falciparum malaria, erythrocyte thiobarbituric acid-reactive substance (ETBAR), and intracellular, membrane and extracellular antioxidants were estimated in 102 cases of P. falciparum malaria and 50 control subjects. The mean concentration of ETBAR was significantly higher (P < 0.001) and many of the antioxidants were significantly lower in patients than controls. Among the erythrocyte antioxidants, catalase, reduced glutathione (GSH) and tocopherol were significantly lower in the patients (P < 0.05, 0.001, 0.001, respectively). Erythrocyte superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) were not reduced to a statistically significant level. Similarly, the plasma antioxidants ascorbate and albumin were significantly lower (P < 0.001) but not urate. ETBAR correlated inversely with erythrocyte GSH and tocopherol (P < 0.001), and plasma ascorbate and albumin (P < 0.001) but not with the erythrocyte enzymic antioxidants. However, on multiple regression analysis only tocopherol correlated strongly with ETBAR, followed by GSH and plasma ascorbate. ETBAR also correlated well with haemolytic indices such as haemoglobin, plasma unconjugated bilirubin and haptoglobin concentrations (P < 0.001, for all). On follow-up after 2 weeks, ETBAR and different antioxidants reached near control levels. These observations indicate an enhanced oxidative stress on erythrocytes in acute falciparum malaria that may contribute substantially to haemolysis and anaemia.