Effects of Sodium Channel-Targeted Conotoxins on Catecholamine Release in Adrenal Chromaffin Cells
Effects of Sodium Channel-Targeted Conotoxins on Catecholamine Release in Adrenal Chromaffin Cells
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钠通道靶向芋螺毒素对肾上腺嗜铬细胞儿茶酚胺释放的影响
DOI：
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发表时间：
2008
期刊：
影响因子：
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通讯作者：
K. Kumakura
中科院分区：
文献类型：
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作者：
E. C. Jimenez;N. Sasakawa;K. Kumakura
Veratridine 是一种钠通道失活抑制剂，可导致 Ca2+ 流入和儿茶酚胺 (CA) 释放。肾上腺嗜铬细胞用钠通道靶向的芋螺毒素、μO-MrVIA 和 μ-PIIIA 处理，然后应用完全被河豚毒素 (TTX) 抑制的 20 μM 藜芦定。 2.5 μM 时，μO-MrVIA 完全消除了藜芦定诱导的 TTX 敏感 CA 释放。另一方面，μ-PIIIA (0.5-2.5 μM) 是一种效力较低的抑制剂；抑制稳定在约 60%。结果表明，在肾上腺嗜铬细胞中，TTX敏感的钠通道可分为两类：（1）对μO-MrVIA和μ-PIIIA均敏感（60％），（2）对μO-MrVIA敏感但对μ-PIIIA耐药（40％）。芋螺毒素 μO-MrVIA 和 μ-PIIIA 可用于区分结构不同的钠通道。具体而言，PIIIA 可用于确定各种类型细胞分化过程中钠通道亚型共表达的功能意义和调节。
Veratridine, a sodium channel inactivation inhibitor causes Ca2+ influx and catecholamine (CA) release. Adrenal chromaffin cells were treated with sodium channel-targeted conotoxins, μO-MrVIA and μ-PIIIA, prior to the application of 20 μM veratridine that was completely inhibited by tetrodotoxin (TTX). At 2.5 μM, μO-MrVIA completely abolished the veratridineinduced TTX-sensitive CA release. On the other hand, μ-PIIIA (0.5 2.5 μM) is a less potent inhibitor; the inhibition leveled off at ~ 60 %. The results indicate that in adrenal chromaffin cells, the TTX-sensitive sodium channels can be classified into two categories: (1) sensitive to both μO-MrVIA and μ-PIIIA (60 %), and (2) sensitive to μO-MrVIA but resistant to μ-PIIIA (40 %). Conotoxins μO-MrVIA and μ-PIIIA may be used in discriminating structurally different sodium channels. Specifically, PIIIA may be useful in determining the functional significance and regulation of co-expression of sodium channel subtypes during differentiation in various types of cells.
MicroO-芋螺毒素 MrVIA 抑制哺乳动物钠通道，但不是通过位点 I。
DOI：
10.1152/jn.1996.76.3.1423
发表时间：
1996
期刊：
Journal of neurophysiology.
影响因子：
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作者：
Terlau,H;Stocker,M;Shon,KJ;McIntosh,JM;Olivera,BM
通讯作者：
Olivera,BM