Development of a genetic linkage map and identification of homologous linkage groups in sweetpotato using multiple-dose AFLP markers
Development of a genetic linkage map and identification of homologous linkage groups in sweetpotato using multiple-dose AFLP markers
复制标题
DOI：
10.1007/s11032-007-9150-6
复制
发表时间：
2008-05-01
影响因子：
3.1
通讯作者：
Sosinski, Bryon R.
中科院分区：
文献类型：
--
作者：
Cervantes-Flores, Jim C.;Yencho, G. Craig;Sosinski, Bryon R.
尽管甘薯作为一种粮食作物在世界范围内具有重要意义，但与大多数其他主要作物相比，甘薯的基因组研究微乎其微。甘薯遗传连锁图谱的建立将提供关于甘薯这一重要物种基因组结构的有价值的信息，育种家可以利用这些信息来加速将所需性状导入育种系。我们开发了一个由240个个体组成的图谱群体，这些个体是奶油肉质的非洲地方品种坦桑尼亚和肉质橙色的美国甘薯品种博雷加德之间的杂交。利用扩增片段长度多态性(AFLP)标记构建了该群体的遗传连锁图谱。总共对1944个(坦桑尼亚)和1751个(博雷加德)AFLP标记进行了评分，其中1511个和1303个分别是单剂量标记。利用JoinMap 3.0和MapMaker/EXP 3.0的组合开发了分别由86个和90个连锁组组成的框架图，分别用于‘坦桑尼亚’和‘博雷加德’。总共有947个单剂标记被放置在“坦桑尼亚”的最终框架链接图中。连锁图谱大小为5792 cM，标记间平均距离为4.5 cM。总共有726个单剂标记物被放置在最终的框架图中。连锁图谱长度为5276 cM，标记间平均距离为4.8 cM。使用双重和三剂量标记来识别图谱中相应的同源基团。我们的研究支持甘薯是同源多倍体的假说。本研究中一些标记在不同剂量下的扭曲分离表明甘薯中存在一定的优先配对现象。然而，由于观察到的标记分离比例以及单倍体标记与多剂量标记的比例，可以排除甘薯严格的异源多倍体遗传。
Sweetpotato genomic research is minimal compared to most other major crops despite its worldwide importance as a food crop. The development of a genetic linkage map in sweetpotato will provide valuable information about the genomic organization of this important species that can be used by breeders to accelerate the introgression of desired traits into breeding lines. We developed a mapping population consisting of 240 individuals of a cross between 'Tanzania', a cream-fleshed African landrace, and 'Beauregard', an orange-fleshed US sweetpotato cultivar. The genetic linkage map of this population was constructed using Amplified Fragment Length Polymorphism (AFLP) markers. A total of 1944 ('Tanzania') and 1751 ('Beauregard') AFLP markers, of which 1511 and 1303 were single-dose markers respectively, were scored. Framework maps consisting of 86 and 90 linkage groups for 'Tanzania' and 'Beauregard' respectively, were developed using a combination of JoinMap 3.0 and MAPMAKER/EXP 3.0. A total of 947 single-dose markers were placed in the final framework linkage map for 'Tanzania'. The linkage map size was estimated as 5792 cM, with an average distance between markers of 4.5 cM. A total of 726 single-dose markers were placed in the final framework map for 'Beauregard'. The linkage map length was estimated as 5276 cM, with an average distance between markers of 4.8 cM. Duplex and triple-dose markers were used to identify the corresponding homologous groups in the maps. Our research supports the hypothesis that sweetpotato is an autopolyploid. Distorted segregation in some markers of different dosages in this study suggests that some preferential pairing occurs in sweetpotato. However, strict allopolyploid inheritance in sweetpotato can be ruled out due to the observed segregation ratios of the markers, and the proportion of simplex to multiple-dose markers.