Putting structure into context: fitting of atomic models into electron microscopic and electron tomographic reconstructions
Putting structure into context: fitting of atomic models into electron microscopic and electron tomographic reconstructions
复制标题
DOI：
10.1016/j.ceb.2011.11.002
复制
发表时间：
2012-02-01
影响因子：
7.5
通讯作者：
Volkmann, Niels
中科院分区：
文献类型：
--
作者：
Volkmann, Niels
一个完整的理解复杂的动态细胞过程，如细胞迁移或细胞粘附，需要整合原子水平的结构信息到更大的细胞背景。虽然细胞水平上的直接原子水平信息仍然无法获得，但电子显微镜、电子断层扫描及其相关的计算图像处理方法现在已经成熟到可以在纳米尺度上对亚细胞结构进行三维成像的程度。通过其他手段获得的原子分辨率信息可以与这些数据相结合，以获得在其细胞背景下的大型大分子组装的三维模型。本文综述了该领域的一些最新进展。
A complete understanding of complex dynamic cellular processes such as cell migration or cell adhesion requires the integration of atomic level structural information into the larger cellular context. While direct atomic-level information at the cellular level remains inaccessible, electron microscopy, electron tomography and their associated computational image processing approaches have now matured to a point where sub-cellular structures can be imaged in three dimensions at the nanometer scale. Atomic-resolution information obtained by other means can be combined with this data to obtain three-dimensional models of large macromolecular assemblies in their cellular context. This article summarizes some recent advances in this field.