Complementary transcriptomic and proteomic analyses reveal regulatory mechanisms of milk protein production in dairy cows consuming different forages.
Complementary transcriptomic and proteomic analyses reveal regulatory mechanisms of milk protein production in dairy cows consuming different forages.
复制标题
互补的转录组学和蛋白质组学分析揭示了消耗不同饲料的奶牛乳蛋白生产的调节机制
DOI：
10.1038/srep44234
复制
发表时间：
2017-03-14
影响因子：
4.6
通讯作者：
Liu H
中科院分区：
文献类型：
--
作者：
Dai W;Chen Q;Wang Q;White RR;Liu J;Liu H
饲草在奶牛产奶量中起着至关重要的作用，然而，对饲喂不同基础饲料类型的高日粮奶牛的牛乳合成调控机制还不是很清楚。本研究用稻草(RS，低品质)和紫花苜蓿干草(AH，优质)日粮饲喂奶牛，利用iTRAQ蛋白质组学技术，利用RNA-Seq转录方法，探讨饲料品质对奶牛产奶量调控分子机制的影响。共有554个转录本(423个上调，131个下调)和517个蛋白质(231个上调，286个下调)在两组乳腺中差异表达。相关分析表明，有7个蛋白质(6个上调，1个下调)具有一致的mRNA表达。对差异表达的转录本/蛋白质的功能分析表明，能量和脂肪酸代谢能力的增强、蛋白质降解的增加、蛋白质合成的减少、氨基酸代谢的降低和细胞生长的抑制与RS的消耗有关。结果表明，食用RS日粮的奶牛可能抑制了牛奶蛋白质的合成，因为这些动物的蛋白质合成能力降低，蛋白质分解能力增强，能量产生效率低下，细胞生长减少。评估基于RS和AH的日粮的额外工作可能有助于更好地分离分子对哺乳期低营养利用率的适应。
Forage plays a critical role in the milk production of dairy cows; however, the mechanisms regulating bovine milk synthesis in dairy cows fed high forage rations with different basal forage types are not well-understood. In the study, rice straw (RS, low-quality) and alfalfa hay (AH, high-quality) diets were fed to lactating cows to explore how forage quality affected the molecular mechanisms regulating milk production using RNA-seq transcriptomic method with iTRAQ proteomic technique. A total of 554 transcripts (423 increased and 131 decreased) and 517 proteins (231 up-regulated and 286 down-regulated) were differentially expressed in the mammary glands of the two groups. The correlation analysis demonstrated seven proteins (six up-regulated and one down-regulated) had consistent mRNA expression. Functional analysis of the differentially expressed transcripts/proteins suggested that enhanced capacity for energy and fatty acid metabolism, increased protein degradation, reduced protein synthesis, decreased amino acid metabolism and depressed cell growth were related to RS consumption. The results indicated cows consuming RS diets may have had depressed milk protein synthesis because these animals had decreased capacity for protein synthesis, enhanced proteolysis, inefficient energy generation and reduced cell growth. Additional work evaluating RS- and AH-based rations may help better isolate molecular adaptations to low nutrient availability during lactation.