Detection and isolation of highly pathogenic H5N1 avian influenza A viruses from blow flies collected in the vicinity of an infected poultry farm in Kyoto, Japan, 2004
Detection and isolation of highly pathogenic H5N1 avian influenza A viruses from blow flies collected in the vicinity of an infected poultry farm in Kyoto, Japan, 2004
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DOI：
10.4269/ajtmh.2006.75.327
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发表时间：
2006-08-01
影响因子：
3.3
通讯作者：
Kobayashi, Mutsuo
中科院分区：
文献类型：
--
作者：
Sawabe, Kyoko;Hoshino, Keita;Kobayashi, Mutsuo
在2004年京都县丹波镇发生的高致病性禽流感爆发期间，从家禽养殖场半径2.3公里范围内的6个地点共收集了926只苍蝇。采用逆转录-聚合酶链反应（RT-PCR）技术，从黑须丽蝇（Calliphoraigribabis）和灰腹丽蝇（Aldrichinagrahami）的肠道、生殖器和内脏中检测到H5亚型流感病毒的基质蛋白（M）和血凝素（HA）基因。HA基因在HA裂解位点编码多个碱性氨基酸，表明该病毒为高致病性毒株。根据鸡胚分离病毒的M、HA和神经氨酸酶（NA）片段的全长序列，从C.黑须蝇（A/blow fly/京都/93/2004）被表征为H5 N1亚型甲型流感病毒，并且显示在所有三个RNA区段中与来自鸡（A/chicken/京都/3/2004）和乌鸦（A/crows/京都/53/2004）的毒株具有> 99.9%的同一性，所述毒株是在2004年京都的该爆发期间衍生的。我们的研究结果表明，这是可能的，打击苍蝇可能成为一个机械传输的H5 N1流感病毒。
During the outbreak of highly pathogenic avian influenza that occurred in Tamba Town, Kyoto Prefecture in 2004, a total of 926 flies were collected from six sites within a radius of 2.3 km from the poultry farm. The H5 influenza A virus genes were detected from the intestinal organs, crop, and gut of the two blow fly species, Calliphora nigribarbis and Aldrichina grahami, by reverse transcription-polymerase chain reaction for the matrix protein (M) and hemagglutinin (HA) genes. The HA gene encoding multiple basic amino acids at the HA cleavage site indicated that this virus is a highly pathogenic strain. Based on the full-length sequences of the M, HA, and neuraminidase (NA) segments of virus isolates through embryonated chicken eggs, the virus from C. nigribarbis (A/blow fly/Kyoto/93/2004) was characterized as H5N1 subtype influenza A virus and shown to have > 99.9% identities in all three RNA segments to a strain from chickens (A/chicken/Kyoto/3/2004) and crows (A/crows/Kyoto/53/2004) derived during this outbreak period in Kyoto in 2004. Our results suggest it is possible that blow flies could become a mechanical transmitter of H5N1 influenza virus.