Antioxidant and hepatoprotective actions of medicinal herb, Terminalia catappa L. from Okinawa Island and its tannin corilagin
Antioxidant and hepatoprotective actions of medicinal herb, Terminalia catappa L. from Okinawa Island and its tannin corilagin
复制标题
DOI：
10.1016/j.phymed.2006.12.012
复制
发表时间：
2007-11-01
期刊：
影响因子：
7.9
通讯作者：
Aniya, Y.
中科院分区：
文献类型：
--
作者：
Kinoshita, S.;Inoue, Y.;Aniya, Y.
使用叶子提取物和分离的抗氧化剂在体外和体内评估了从冲绳岛采集的榄仁树的抗氧化和保肝作用。 T catappa 叶的水提取物对 1,1-二苯基-2-三硝基苯肼和超氧阴离子 (O-2(-)) 具有很强的自由基清除作用。从 T catappa 中分离得到活性成分 Chebulagacid 和 Corilagin。两种抗氧化剂均对 O-2(-) 和过氧自由基具有很强的清除作用，并且还能抑制佛波醇-12-肉豆蔻酸酯乙酸酯刺激的白细胞产生活性氧。半乳糖胺（GalN，600mg/kg，s.c.）和脂多糖（LPS，0.5μg/kg，i.p.）诱导的大鼠肝毒性，表现为血清丙氨酸氨基转移酶、天冬氨酸氨基转移酶和谷胱甘肽 S-转移酶（GST）活性升高，当草药提取物时显着降低 在 GalN/LPS 治疗前给大鼠腹腔注射柯里拉京或柯里拉京。由 GalN/LPS 处理引起的线粒体中自由基形成和脂质过氧化的增加也通过用草药/柯里拉京预处理而减少。此外，用草药/柯里拉京预处理可以预防用 GalN/LPS 处理观察到的细胞凋亡事件，例如 DNA 断裂和肝脏中 caspase-3 活性的增加。这些结果表明，T catappa 提取物及其抗氧化剂 Corilagin 通过抑制氧化应激和细胞凋亡，对 GaIN/LPS 诱导的肝损伤具有保护作用。 (C) 2006 爱思唯尔有限公司。版权所有。
The antioxidant and hepatoprotective actions of Terminalia catappa L. collected from Okinawa Island were evaluated in vitro and in vivo using leaves extract and isolated antioxidants. A water extract of the leaves of T catappa showed a strong radical scavenging action for 1,1-diphenyl-2-picrylhydrazyl and superoxide (O-2(-)) anion. Chebulagic acid and corilagin were isolated as the active components from T catappa. Both antioxidants showed a strong scavenging action for O-2(-) and peroxyl radicals and also inhibited reactive oxygen species production from leukocytes stimulated by phorbol-12-myristate acetate. Galactosamine (GalN, 600mg/kg, s.c.,) and lipopolysaccharide (LPS, 0.5 mu g/kg, i.p.)-induced hepatotoxicity of rats as seen by an elevation of serum alanine aminotransferase, aspartate aminotransferase and glutathione S-transferase (GST) activities was significantly reduced when the herb extract or corilagin was given intraperitoneally to rats prior to GalN/LPS treatment. Increase of free radical formation and lipid peroxidation in mitochondria caused by GalN/LPS treatment were also decreased by pretreatment with the herb/corilagin. In addition, apoptotic events such as DNA fragmentation and the increase in caspase-3 activity in the liver observed with GalN/LPS treatment were prevented by the pretreatment with the herb/corilagin. These results show that the extract of T catappa and its antioxidant, corilagin are protective against GaIN/LPS-induced liver injury through suppression of oxidative stress and apoptosis. (C) 2006 Elsevier GmbH. All rights reserved.