Comparative analysis of the S-intergenic region in class-II S haplotypes of self-incompatible Brassica rapa (syn. campestris)
Comparative analysis of the S-intergenic region in class-II S haplotypes of self-incompatible Brassica rapa (syn. campestris)
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DOI：
10.1266/ggs.81.63
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发表时间：
2006-02-01
影响因子：
1.1
通讯作者：
Watanabe, Masao
中科院分区：
文献类型：
--
作者：
Kakizaki, Tomohiro;Takada, Yoshinobu;Watanabe, Masao
在芸苔属自交不亲和性 (SI) 系统中，花粉决定簇 SP11 参与花粉 SI 表型的显性/隐性关系。为了深入了解 SP11 周围的基因组结构以及显性/隐性关系的机制，我们对三种花粉隐性 II S 类单倍型中包含 SP11 和 SRK 基因的基因组区域进行了表征。 S基因的转录方向在II类S单倍型中完全保守。然而，SP11和SRK之间的区域（S-基因间区域）具有高度多态性，没有短重复序列。此外，我们发现短重复序列与SP11的5'上游区域之间存在序列相似性。人们发现这种序列相似性可能与通过染色质结构的变化表达优势关系有关。
In the Brassica self-incompatibility (SI) system, a pollen determinant, SP11, is involved in dominance/recessive relationships in pollen SI phenotypes. In order to gain some insights into the genomic structure around the SP11 and the mechanisms that give dominance/recessive relationships, we characterized the genomic region containing SP11 and SRK genes in three pollen recessive class-II S haplotypes. The direction of transcription of S genes was completely conserved among class-II S haplotypes. However, the region between SP11 and SRK (S-intergenic region) was highly polymorphic without short repetitive sequences. In addition, we found a sequence similarity between the short repetitive sequence and 5'-upstream region of SP11. This sequence similarity was found to be potentially related to the expression of dominance relationships through the change of chromatin structure.