Tissue activity and cellular localization of human semicarbazide-sensitive amine oxidase
Tissue activity and cellular localization of human semicarbazide-sensitive amine oxidase
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DOI：
10.1177/002215540104900208
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发表时间：
2001-02-01
影响因子：
3.2
通讯作者：
Mahy, N
中科院分区：
文献类型：
--
作者：
Andrés, N;Lizcano, JM;Mahy, N
氨基脲敏感胺氧化酶（SSAO）广泛分布于高度血管化的哺乳动物组织中，代谢内源性和外源性芳香和脂肪族单胺。为了评估其在人体中的生理作用是否仅限于氧化，我们使用免疫组织化学方法检测了SSAO在人体外周组织（肾上腺、十二指肠、心脏、肾脏、肺、肝脏、胰腺、脾脏、甲状腺和血管）中的细胞定位，并分析了其亚细胞定位。该结果与在相同样品中测定的特异性活性一致，并参考单胺氧化酶A和b的组织分布进行了讨论。与单胺氧化脱胺作用一起，SSAO的细胞定位表明，在大多数人体外周组织中，SSAO可能通过生成H2O2参与生理过程的调节。
Semicarbazide-sensitive amine oxidase (SSAO), widely distributed in highly vascularized mammalian tissues, metabolizes endogenous and xenobiotic aromatic and aliphatic monoamines. To assess whether its physiological role in humans is restricted to oxidation, we used an immunohistochemical approach to examine the cellular localization of SSAO in human peripheral tissues (adrenal gland, duodenum, heart, kidney, lung, liver, pancreas, spleen, thyroid gland, and blood vessels) and also analyzed its subcellular localization. The results are in agreement with the specific activities also determined in the same samples and are discussed with reference to the tissue distribution of monoamine oxidase A and B. Together with the oxidative deamination of monoamines, SSAO cellular localization indicates that, in most human peripheral tissues, it might participate in the regulation of physiological processes via H2O2 generation.