Evaluation of Anti proliferative Activities and Action Mechanisms of Extracts from Two Species of Ganoderma on Tumor Cell Lines
Evaluation of Anti proliferative Activities and Action Mechanisms of Extracts from Two Species of Ganoderma on Tumor Cell Lines
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DOI：
10.1021/jf900011f
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发表时间：
2009-04-22
影响因子：
6.1
通讯作者：
Li, Shao-Ping
中科院分区：
文献类型：
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作者：
Liu, Yu-Wei;Gao, Jian-Li;Li, Shao-Ping
以人乳腺癌（MCF-7和MDA-MB-231）、人肝癌（HepG 2）和人髓系白血病（HL-60）细胞为实验对象，研究了灵芝（Ganoderma）、灵芝属（Ganoderma）和灵芝属（Ganoderma）2种植物的乙醇提取物对肿瘤细胞的抑制增殖活性。lucidum和G. sinense）的根际土壤进行了研究。虽然两种提取物均具有一定的抗增殖活性，但其核苷、三萜、甾醇等化学成分有显著差异。采用细胞凋亡检测和细胞周期分析进一步研究了它们对MDA-MB-231细胞的作用。结果表明，两种提取物均能通过改变线粒体跨膜去极化而诱导细胞凋亡，但未检测到三萜类化合物。中国的此外，G. lucidum和G.结果表明，紫云英能使细胞周期阻滞在不同的时期。结果表明，两种甘草的乙醇提取物对番茄红素的含量均无显著影响。lucidum和G. sinense具有抗肿瘤增殖作用，其机制可能与细胞凋亡和细胞周期阻滞有关，除三萜类化合物外，还可能与甾醇和/或核苷类化合物有关。
The antiproliferative activities on tumoral cells, namely, human breast cancer (MCF-7 and MDA-MB-231), hepatoma (HepG2) and myeloid leukemia (HL-60), of ethanolic extracts from two species of Ganoderma, G. lucidum and G. sinense, were investigated. Though both extracts had certain antiproliferative activities, their chemical characteristics, including nucleosides, triterpenoids and sterols, were significantly different. Their effects on MDA-MB-231 cells were further studied using apoptotic detection and cell cycle analyses. As a result, both had apoptosis induction through the alternation of mitochondrial transmembrane depolarization, though no triterpenoids were detected in ethanolic extract of G. sinense. Furthermore, the two extracts from G. lucidum and G. sinense could arrest cell cycle at different phases. This study showed that ethanol extracts of both G. lucidum and G. sinense have antitumoral proliferation effect through both apoptosis pathway and cell cycle arrest effect, and some other compounds such as sterols and/or nucleosides may contribute to their activity besides triterpenoids.