Human neural stem cells: electrophysiological properties of voltage-gated ion channels
Human neural stem cells: electrophysiological properties of voltage-gated ion channels
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DOI：
10.1097/00001756-200208070-00020
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发表时间：
2002-08-07
期刊：
影响因子：
1.7
通讯作者：
Min, CK
中科院分区：
文献类型：
--
作者：
Cho, T;Bae, JH;Min, CK
我们的特点是在永生化人神经干细胞系，HBI.F3细胞的膜电流的档案，使用全细胞膜片钳技术。用含v-myc基因的逆转录病毒载体从胚胎人端脑原代细胞培养物中获得的人神经干细胞系表达神经干细胞的细胞类型特异性标志物巢蛋白。人神经干细胞表达外向和内向K+电流，没有Na+电流的证据。外向延迟整流型K+电流密度为1.8+/-0.015 nA/pF，内向整流型K+电流密度为0.37+/-0.012 nA/pF（在30 mM [K+]（o）时）。为了诱导神经干细胞的神经元分化，将神经源性转录因子NeuroD的全长编码区转染到HBI.F3细胞中。NeuroD诱导的Na+电流密度为0.042+/-0.011 nA/pF。两种类型的K+电流的存在和NeuroD诱导的Na+电流的表达似乎反映了人神经干细胞的特征性生理特征。
We have characterized the profile of membrane currents in an immortalized human neural stem cell line, HBI.F3 cells, using whole-cell patch clamp technique. Human neural stem cell line generated from primary cell cultures of embryonic human telencephalon using a replication-incompetent retroviral vector containing v-myc expresses nestin, a cell type-specific marker for neural stem cells. The human neural stem cells expressed both outward and inward K+ currents with no evidence for Na+ currents. The density of the outward, delayed rectifying type K+ current was 1.8+/-0.015 nA/pF, and that of the inwardly rectifying K+ current was 0.37+/-0.012 nA/pF (at 30 mM of [K+](o)). In order to induce neuronal differentiation of the neural stem cells, a full-length coding region of NeuroD, a neurogenic transcription factor, was transfected into HBI.F3 cells. Introduction of NeuroD induced expression of Na+ currents with the current density of 0.042+/-0.011 nA/pF. The presence of two types of K+ currents and expression of Na+ currents induced by NeuroD appear to reflect the characteristic physiological features of human neural stem cells.