Inhibition of ADP-induced platelet aggregation by reduced factor Xa.
Inhibition of ADP-induced platelet aggregation by reduced factor Xa.
复制标题
通过还原因子 Xa 抑制 ADP 诱导的血小板聚集。
DOI：
10.1111/j.1432-1033.1983.tb07704.x
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发表时间：
1983
期刊：
影响因子：
--
通讯作者：
Colman,RW
中科院分区：
文献类型：
--
作者：
Sinha,AK;Colman,RW
用不溶性前列腺素 E1 (PGE1) 己基琼脂糖衍生物处理凝血因子 Xa 会导致蛋白质分子中产生两个巯基。还原因子 Xa 被发现是 ADP 诱导的血小板聚集和血栓素 A2 合成的有效抑制剂。与抑制血栓素形成相反，还原型因子Xa对PGE 2 的形成没有影响，表明还原型因子Xa可能选择性地抑制血栓素合成酶。与氧化型谷胱甘肽一起孵育可逆转先前暴露于不溶性激素的 Xa 因子的抑制活性。可溶性 PGE1 也会降低因子 Xa，但速度比不溶性 PGE1 慢。通过氧化还原染料测试，PGE1 还表现出还原能力。二硫苏糖醇对 Xa 因子的还原也将凝血因子转化为血小板聚集和血栓素 A2 形成的抑制剂。这些实验表明Xa因子的减少导致抑制ADP诱导的血小板聚集的分子的可逆改变。还原因子 Xa 的这种作用可能是通过抑制血栓素 A2 合成来介导的。
Treatment of blood coagulation factor Xa with insolubilized hexyl‐agarose derivative of prostaglandin E1(PGE1) results in the generation of two sulfhydryl groups in the protein molecule. The reduced factor Xa was found to be a potent inhibitor of platelet aggregation and thromboxane A2synthesis induced by ADP. In contrast to the inhibition of thromboxane formation, the reduced factor Xa had no effect on the formation of PGE2indicating that thromboxane synthetase might be selectively inhibited by the reduced factor Xa. Incubation with oxidized glutathione reversed the inhibitory activity of factor Xa previously exposed to the insolubilized hormone. Soluble PGE1also reduces factor Xa, but more slowly than the insolubilized PGE1. PGE1also exhibits reducing ability as tested with redox dyes.Reduction of factor Xa by dithiothreitol also transformed the coagulation factor into an inhibitor of platelet aggregation and thromboxane A2formation. These experiments indicate that reduction of factor Xa leads to a reversible alteration of the molecule which inhibits platelet aggregation induced by ADP. This effect of reduced factor Xa is probably mediated through the inhibition of thromboxane A2synthesis.