Molecular interaction and cellular studies on combination photodynamic therapy with rutoside for melanoma A375 cancer cells: an in vitro study.
Molecular interaction and cellular studies on combination photodynamic therapy with rutoside for melanoma A375 cancer cells: an in vitro study.
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DOI：
10.1186/s12935-020-01616-x
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发表时间：
2020
影响因子：
5.8
通讯作者：
Chamani E
中科院分区：
文献类型：
--
作者：
Khorsandi K;Hosseinzadeh R;Chamani E
黑色素瘤作为皮肤癌的一种，与高死亡率相关。因此，早期诊断和有效的手术治疗是非常重要的。光动力疗法（PDT）涉及通过特定波长的光激活光敏剂，所述光与氧相互作用并产生单线态氧分子或活性氧物质（ROS），其可导致肿瘤细胞死亡。此外，植物化合物的应用也是预防和治疗各种癌症的主要途径之一。酚类化合物是一类重要的天然抗氧化剂，具有重要的生物学功能，如抗癌作用。以前认为黄酮类化合物如芸香苷可以作为促氧化剂或抗氧化剂。因此，在本研究中，我们的目的是研究芸香苷的联合治疗与亚甲蓝（MB）辅助光动力学治疗（PDT），使用红色光源（660 nm;功率密度：30 mW/cm 2）对A375人黑色素瘤癌细胞的影响。为此，用MB-PDT和芸香苷处理A375人黑素瘤癌细胞系。在进行处理后，还测定了克隆形成细胞存活率、MTT测定和细胞死亡机制。随后，在芸香苷处理和光动力学治疗（PDT）后，细胞周期和细胞内活性氧（ROS）的产生进行了测定。结果表明，MB-PDT和芸香苷对A375黑色素瘤细胞具有较好的细胞毒和抗增殖作用，而对HDF人皮肤成纤维细胞的细胞毒作用不明显。MB-PDT联合芸香苷诱导人黑色素瘤细胞凋亡及细胞周期阻滞MB PDT和芸香苷处理后A375细胞内ROS增加。结果表明，MB-PDT和芸香苷可以被认为是黑色素瘤的联合治疗的新方法。
Melanoma as a type of skin cancer, is associated with a high mortality rate. Therefore, early diagnosis and efficient surgical treatment of this disease is very important. Photodynamic therapy (PDT) involves the activation of a photosensitizer by light at specific wavelength that interacts with oxygen and creates singlet oxygen molecules or reactive oxygen species (ROS), which can lead to tumor cell death. Furthermore, one of the main approches in the prevention and treatment of various cancers is plant compounds application. Phenolic compounds are essential class of natural antioxidants, which play crucial biological roles such as anticancer effects. It was previously suggested that flavonoid such as rutoside could acts as pro-oxidant or antioxidant. Hence, in this study, we aimed to investigate the effect of rutoside on the combination therapy with methylene blue (MB) assisted by photodynamic treatment (PDT) using red light source (660 nm; power density: 30 mW/cm2) on A375 human melanoma cancer cells. For this purpose, the A375 human melanoma cancer cell lines were treated by MB-PDT and rutoside. Clonogenic cell survival, MTT assay, and cell death mechanisms were also determined after performing the treatment. Subsequently, after the rutoside treatment and photodynamic therapy (PDT), cell cycle and intracellular reactive oxygen species (ROS) generation were measured. The obtained results showed that, MB-PDT and rutoside had better cytotoxic and antiprolifrative effects on A375 melanoma cancer cells compared to each free drug, whereas the cytotoxic effect on HDF human dermal fibroblast cell was not significant. MB-PDT and rutoside combination induced apoptosis and cell cycle arrest in the human melanoma cancer cell line. Intracellular ROS increased in A375 cancer cell line after the treatment with MB-PDT and rutoside. The results suggest that, MB-PDT and rutoside could be considered as novel approaches as the combination treatment of melanoma cancer.