Development of an integrative database with 499 novel microsatellite markers for Macaca fascicularis
Development of an integrative database with 499 novel microsatellite markers for Macaca fascicularis
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DOI：
10.1186/1471-2156-10-24
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发表时间：
2009-06-05
期刊：
影响因子：
2.9
通讯作者：
Terao, Keiji
中科院分区：
文献类型：
--
作者：
Higashino, Atsunori;Osada, Naoki;Terao, Keiji
工作背景：食蟹猴（Macacafascicularis）是遗传病连锁研究的宝贵资源，但其微卫星标记不足。在遗传学研究中，定位基因的先决条件是在靶生物中开发一组全基因组的微卫星标记。全基因组序列及其注释也有助于鉴定致病突变的标志物。本研究的目的是建立数百个微卫星标记，并开发一个综合的食蟹猴基因组数据库，包括标记和基因信息，将是有用的，为进一步的遗传analyses in this species.Results的各种数据集：我们调查了食蟹猴的多态性水平为671微卫星标记，所涵盖的我们建立的细菌人工染色体（BAC）克隆。499（74.4%）的标记被发现是多态性使用标准PCR分析。10只食蟹猴在这些多态位点上的平均等位基因数和平均期望杂合度分别为8.20和0.75。结论：获得了猕猴基因组BAC克隆和新的微卫星标记，并与食蟹猴cDNA数据库（QFbase）进行了连锁。我们新的微卫星标记集和基因组数据库将是有价值的综合资源，在分析遗传疾病的食蟹猴。
Background: Cynomolgus macaques (Macaca fascicularis) are a valuable resource for linkage studies of genetic disorders, but their microsatellite markers are not sufficient. In genetic studies, a prerequisite for mapping genes is development of a genome-wide set of microsatellite markers in target organisms. A whole genome sequence and its annotation also facilitate identification of markers for causative mutations. The aim of this study is to establish hundreds of microsatellite markers and to develop an integrative cynomolgus macaque genome database with a variety of datasets including marker and gene information that will be useful for further genetic analyses in this species.Results: We investigated the level of polymorphisms in cynomolgus monkeys for 671 microsatellite markers that are covered by our established Bacterial Artificial Chromosome (BAC) clones. Four hundred and ninety-nine (74.4%) of the markers were found to be polymorphic using standard PCR analysis. The average number of alleles and average expected heterozygosity at these polymorphic loci in ten cynomolgus macaques were 8.20 and 0.75, respectively.Conclusion: BAC clones and novel microsatellite markers were assigned to the rhesus genome sequence and linked with our cynomolgus macaque cDNA database (QFbase). Our novel microsatellite marker set and genomic database will be valuable integrative resources in analyzing genetic disorders in cynomolgus macaques.