REDIFFERENTIATION OF MOSS PROTONEMATA - AN EXPERIMENTAL AND IMMUNOFLUORESCENCE STUDY OF BROOD CELL-FORMATION
REDIFFERENTIATION OF MOSS PROTONEMATA - AN EXPERIMENTAL AND IMMUNOFLUORESCENCE STUDY OF BROOD CELL-FORMATION
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DOI：
10.1139/b93-183
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发表时间：
1993-11-01
期刊：
影响因子：
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通讯作者：
DUCKETT, JG
中科院分区：
文献类型：
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作者：
GOODE, JA;STEAD, AD;DUCKETT, JG
卵球形或球形的育卵细胞的形成，从绿丝膨胀和对称的亚顶端分裂之前的phragmosomes，但不是preprophase带，被描述在藓类立碗藓，Dicranoweisia cirata，Bryum tenuisetum，Bryum bicolor，和Rhytidiadelphus loreus。这是一个普遍的现象，发生在原丝体殖民地培养了很长一段时间或允许干燥。它涉及到高度极化的chloronemal细胞与一个净轴向阵列的微管到非极性细胞含有微管没有优先方向的再分化。有些物种的卵细胞变得很厚，即使在干燥的状态下也能保持很长时间的活力。当转移到新的培养基中时，它们立即从没有固定位置的新细丝再生原丝。使用活性炭的实验，它可以防止巢细胞的形成，并将原丝或孢子转移到旧的培养基中，其中巢细胞已经产生以前，表明再分化过程是由原丝释放到培养基中的物质触发。添加脱落酸，一种内源性生长调节剂，在苔藓，导致早熟的育雏细胞形成在年轻的文化，很可能是天然化合物触发他们的发展和诱导他们的耐干燥。
The formation of ovoid or spherical brood cells from chloronemal filaments by swelling and symmetrical subapical divisions preceded by phragmosomes, but not preprophase bands, is described in the mosses Physcomitrella patens, Dicranoweisia cirrata, Bryum tenuisetum, Bryum bicolor, and Rhytidiadelphus loreus. This is a widespread phenomenon that occurs in protonemal colonies cultured for long periods of time or allowed to dry out. It involves the redifferentiation of highly polarized chloronemal cells with a net axial array of microtubules into nonpolar cells containing microtubules with no preferred orientation. Brood cells in some species become thick walled and retain their viability for long periods even in a desiccated state. When transferred to new medium they immediately regenerate protonemata from new filaments of no fixed position. Experiments using activated charcoal, which prevents brood cell formation, and transferring protonemata or spores onto old medium in which brood cells had been produced previously, indicate that the redifferentiation process is triggered by substances released into the medium by protonemata. Addition of abscisic acid, an endogenous growth regulator in mosses, causes precocious brood cell formation in young cultures and is very likely the natural compound triggering their development and inducing their tolerance to desiccation.