MiR-506 Over-Expression Inhibits Proliferation and Metastasis of Breast Cancer Cells.
MiR-506 Over-Expression Inhibits Proliferation and Metastasis of Breast Cancer Cells.
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MiR-506 过度表达抑制乳腺癌细胞的增殖和转移。
DOI：
10.12659/msm.893522
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发表时间：
2015-06-10
期刊：
影响因子：
--
通讯作者：
Lv Z
中科院分区：
文献类型：
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作者：
Yu F;Lv M;Li D;Cai H;Ma L;Luo Q;Yuan X;Lv Z
本研究旨在探讨miR-506与乳腺癌细胞增殖和迁移的关系。将MIR-506模拟物、抑制剂和阴性对照(NC)分别导入乳腺癌细胞MDA-MB-231。采用细胞增殖、细胞计数、集落形成实验和Transwell实验检测乳腺癌细胞的增殖和迁移情况。数据以均值±标准差表示，实验进行了3次。统计分析用SPSS10.0版进行。转染后1d，CCK-8检测显示miR-506抑制剂组细胞增殖显著高于miR-506模拟剂组和NC组(P<；0.05)。在转染后3d或5d，miR-506模拟物组细胞增殖明显受到抑制，而miR-506抑制剂仍显著促进细胞增殖。血细胞计数仪计数结果与细胞增殖结果相似。克隆形成实验显示，miR-506模拟物组的克隆数明显少于miR-506抑制剂组和NC组。Transwell实验显示miR-506模拟物的迁移细胞数明显少于miR-506抑制剂组和NC组。MIR-506过表达显著抑制乳腺癌细胞的增殖、克隆形成和迁移。因此，MIR-506过表达可能能够改善乳腺癌细胞的恶性表型。
This study aimed to investigate the relationship between miR-506 and proliferation and migration of breast cancer cells. MiR-506 mimics, inhibitor, and negative control (NC) were transfected into MDA-MB-231 breast cancer cells. Cell proliferation, cell counting, colony formation assay, and Transwell assay were applied to evaluate the proliferation and migration of breast cancer cells. Data are shown as mean ± standard deviation and the experiment was performed 3 times. Statistical analyses were performed with SPSS version 10.0. At 1 day after transfection, cell proliferation detected by CCK-8 assay was significantly promoted in miR-506 inhibitor when compared with the miR-506 mimics group and the NC group (P<0.05). At 3 days or 5 days after transfection, cell proliferation was markedly inhibited in the miR-506 mimics group, and miR-506 inhibitor was still significantly promoted. Cell counting with a hemocytometer showed similar results to cell proliferation. Colony formation assay showed that the number of colonies in the miR-506 mimics group was significantly smaller than that in the miR-506 inhibitor group and NC group. Transwell assay revealed that the number of migrated cells in miR-506 mimics was markedly smaller than that in the miR-506 inhibitor group and NC group. MiR-506 over-expression significantly inhibits the proliferation, colony formation, and migration of breast cancer cells. miR-506 over-expression may thus be able to improve the malignant phenotype of breast cancer cells.