Identification and Characterization of Long Noncoding RNAs in Ovine Skeletal Muscle
Identification and Characterization of Long Noncoding RNAs in Ovine Skeletal Muscle
复制标题
绵羊骨骼肌中长非编码 RNA 的鉴定和表征
DOI：
10.3390/ani8070127
复制
发表时间：
2018-07-01
期刊：
影响因子：
3
通讯作者：
Wei, Caihong
中科院分区：
文献类型：
--
作者：
Li, Qing;Liu, Ruizao;Wei, Caihong
简单总结 LncRNA 可能在许多生物过程中发挥重要作用。本研究的目的是鉴定特塞尔羊和乌珠穆沁羊骨骼肌中潜在的活性lncRNA并研究其功能。总体而言，发现了2002个lncRNA转录本，其中一些可能与肌肉发育有关。这里获得的发现应该有助于了解 lncRNA 在绵羊肌肉发育中的调节功能，也可能在其他哺乳动物中发挥作用。摘要 长非编码 RNA (lncRNA) 越来越被认为是许多细胞过程中的关键调节因子。然而，关于它们在牲畜中的报道却很少。在这里，我们描述了绵羊骨骼肌中 lncRNA 的鉴定和表征。利用胎儿（第 85 天和 120 天）、新生和成年特塞尔羊和乌珠穆沁羊的腓肠肌构建了 8 个文库。 2002 年鉴定的转录本具有一些共同特征，例如外显子数量、长度和分布。我们还在这些 lncRNA 转录本附近发现了一些编码基因，这些基因与转录调控和发育相关过程特别相关，表明 lncRNA 与肌肉发育相关。此外，在不同品种同一阶段文库之间的两两比较中，共有967个转录本存在差异表达，但只有15个差异表达的lncRNA是所有阶段共有的。其中，我们发现TCONS_00013201在乌珠穆沁样本中表达量较高，而TCONS_00006187和TCONS_00083104在特塞尔样本中表达量较高。此外，乌珠穆沁样本中几乎完全不存在TCONS_00044801、TCONS_00008482和TCONS_00102859。我们的结果表明，这些 lncRNA 表达的差异可能与特塞尔羊和乌珠穆沁羊品种之间观察到的肌肉差异有关。
Simple Summary LncRNAs may play important role in many biological processes. The aims of this research were to identify potential lncRNAs active in skeletal muscle of the Texel and Ujumqin sheep and investigate their functions. Overall, 2002 lncRNA transcripts were found, some of which may be related to muscle development. The findings obtained here should promote understanding of the regulatory functions of lncRNAs in ovine muscle development and potentially also in other mammals. Abstract Long noncoding RNAs (lncRNAs) are increasingly being recognized as key regulators in many cellular processes. However, few reports of them in livestock have been published. Here, we describe the identification and characterization of lncRNAs in ovine skeletal muscle. Eight libraries were constructed from the gastrocnemius muscle of fetal (days 85 and 120), newborn and adult Texel and Ujumqin sheep. The 2002 identified transcripts shared some characteristics, such as their number of exons, length and distribution. We also identified some coding genes near these lncRNA transcripts, which are particularly associated with transcriptional regulation- and development-related processes, suggesting that the lncRNAs are associated with muscle development. In addition, in pairwise comparisons between the libraries of the same stage in different breeds, a total of 967 transcripts were differentially expressed but just 15 differentially expressed lncRNAs were common to all stages. Among them, we found that TCONS_00013201 exhibited higher expression in Ujumqin samples, while TCONS_00006187 and TCONS_00083104 were higher in Texel samples. Moreover, TCONS_00044801, TCONS_00008482 and TCONS_00102859 were almost completely absent from Ujumqin samples. Our results suggest that differences in the expression of these lncRNAs may be associated with the muscular differences observed between Texel and Ujumqin sheep breeds.