Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent
Estimation of total phenolic content and other oxidation substrates in plant tissues using Folin-Ciocalteu reagent
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DOI：
10.1038/nprot.2007.102
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发表时间：
2007-01-01
期刊：
影响因子：
14.8
通讯作者：
Gillespie, Kelly M.
中科院分区：
文献类型：
--
作者：
Ainsworth, Elizabeth A.;Gillespie, Kelly M.
非结构性酚类化合物在植物中具有多种功能，包括作为抗氧化剂。我们描述了一种微板适应比色总酚测定，利用福林-Ciocalteu（F-C）试剂。F-C测定依赖于碱性介质中电子从酚类化合物转移到磷钼酸/磷钨酸络合物，其在765 nm处通过光谱测定。虽然电子转移反应不是特定的酚类化合物，提取过程中消除了约85%的抗坏血酸和其他潜在的干扰化合物。该测定在微量离心管中进行，并在96孔板读数器中进行评估。1 d内至少可处理64个样本。
Non-structural phenolic compounds perform a variety of functions in plants, including acting as antioxidants. We describe a microplate-adapted colorimetric total phenolics assay that utilizes Folin-Ciocalteu (F-C) reagent. The F-C assay relies on the transfer of electrons in alkaline medium from phenolic compounds to phosphomolybdic/phosphotungstic acid complexes, which are determined spectroscopically at 765 nm. Although the electron transfer reaction is not specific for phenolic compounds, the extraction procedure eliminates approximately 85% of ascorbic acid and other potentially interfering compounds. This assay is performed in microcentrifuge tubes and assessed in a 96-well plate reader. At least 64 samples can be processed in 1 d.