Role of N-(carboxymethyl)lysine in the development of ischemic heart disease in type 2 diabetes mellitus.
Role of N-(carboxymethyl)lysine in the development of ischemic heart disease in type 2 diabetes mellitus.
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DOI：
10.3164/jcbn.2007014
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发表时间：
2007-09
影响因子：
2.4
通讯作者：
S Ismail I
中科院分区：
文献类型：
--
作者：
A Ahmed K;Muniandy S;S Ismail I
本研究旨在测定伴和不伴缺血性心脏病（IHD）的2型糖尿病患者的Nε-（羧甲基）赖氨酸（CML）水平，并发现循环CML与2型糖尿病IHD患者的一些临床参数（包括血脂、血红蛋白A1 c（HbA 1c）和丙二醛（MDA））之间的可能关联。血清CML水平通过酶联免疫吸附试验使用多克隆抗CML抗体进行测量。在60例伴2型糖尿病的IHD患者、43例不伴2型糖尿病的IHD患者、64例不伴IHD的2型糖尿病患者和80例性别和年龄匹配的健康受试者中评估CML和MDA的血清水平。在伴或不伴IHD的2型糖尿病患者中进行CML水平与血脂、HbA 1c和脂质过氧化反应之间的相关性研究。各组间血糖、血脂（甘油三酯、总胆固醇、HDL-胆固醇）、MDA、HbA 1c、CML和LDL-胆固醇水平差异有统计学意义（p<0.05）。糖尿病IHD患者CML水平显著高于2型糖尿病但无IHD患者（537.1 ± 86.1vs449.7 ± 54.9，p<0.001）。2型糖尿病合并IHD患者血清CML水平与MDA水平呈正相关，r = 0.338（p = 0.008）。而年龄、HbA 1c、血脂对糖尿病患者CML水平无显著影响（p>0.05）。总之，本研究证实了糖尿病和氧化应激对高水平循环CML的影响。这些结果表明，CML血清水平升高与2型糖尿病IHD的发展有关。
This study aims to determine the levels of Nε-(carboxymethyl)lysine (CML) in patients with Type 2 diabetic patients with and without ischemic heart disease (IHD) and to find for a possible association between circulating CML and a number of clinical parameters including lipids, hemoglobin A1c (HbA1c) and malondialdehyde (MDA) in Type 2 diabetic IHD patients. Serum CML levels were measured by enzyme-linked immunosorbent assay using polyclonal anti-CML antibodies. Serum levels of CML and MDA were assessed in 60 IHD patients with Type 2 diabetes, 43 IHD patients without Type 2 diabetes, 64 Type 2 diabetics without IHD, and 80 sex- and age-matched healthy subjects. Correlations studies between CML levels and lipids, HbA1c, and lipid peroxidation were performed in Type 2 diabetes patients with and without IHD. A statistical significance was observed in the levels of serum glucose, lipids (triglyceride, total cholesterol, HDL-cholesterol), MDA, HbA1c, CML and LDL-cholesterol (p<0.05) between the groups of the study. CML levels were significantly increased in diabetic IHD patients compared with Type 2 diabetes patients but without IHD (537.1 ± 86.1 vs 449.7 ± 54.9, p<0.001). A positive correlation was observed between serum levels of CML and MDA, r = 0.338 (p = 0.008) in Type 2 diabetes patients with IHD. However, age, HbA1c and lipids had no significant influence on CML levels among diabetics (p>0.05). In conclusion, this study demonstrates the effect of both diabetes and oxidative stress on the higher levels of circulating CML. These results showed that increased serum levels of CML are associated with the development of IHD in Type 2 diabetes mellitus.