Molecular Cloning and Phylogenetic Analysis of Canine β-Casein
Molecular Cloning and Phylogenetic Analysis of Canine β-Casein
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犬β-酪蛋白的分子克隆及系统发育分析
DOI：
10.3109/10425170009033246
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
S. Tateyama
中科院分区：
文献类型：
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作者：
Manabu Watanabe;S. Sugano;T. Togashi;J. Imai;K. Uchida;R. Yamaguchi;S. Tateyama
采用简并引物聚合酶链反应（PCR）技术，从犬乳腺组织中扩增出β-酪蛋白cDNA。它编码250个氨基酸的蛋白质，含有β-酪蛋白的保守序列基序。与雪豹（Uncia uncia）同源性最高（55- 62%）。与人的同源性为44-5396，与小鼠的同源性为33 - 42%，与大鼠的同源性为29- 37%，与兔的同源性为43- 53%，与猪的同源性为41 - 48%，与牛的同源性为44- 51%，与绵羊的同源性为44- 50%.北方印迹分析显示，乳腺组织中有1.2kb的mRNA。系统发育分析表明，犬β-酪蛋白与小熊猫、雪豹等动物形成了一个分支，属于食肉动物。
A canine β-casein cDNA was isolated from mammary tissue by polymerase chain reaction (PCR) using degenerate primers. It encodes 250 amino acids protein containing the conserved sequence motif of β-casein. It showed the highest homology with snow-leopard (Uncia uncia) (55–62 % identity). It also showed 44–5396 identity with human, 3342 % identity with mouse, 29–37 % identity with rat, 43–53 % identity with rabbit, 4148 % identity with pig, 44–51 % identity with cattle and 44–50 % identity with sheep. A 1.2-kb mRNA was detected in mammary tissue by Northern blot analysis. Phylogenetic analysis revealed that canine β-casein formed a branch with lesser panda and snow leopard, which were grouped into carnivore.