Rhizobium oryziradicis sp nov., isolated from rice roots
Rhizobium oryziradicis sp nov., isolated from rice roots
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从稻根中分离出的米根瘤菌 (Rhizobium oryziradicis sp nov.)
DOI：
10.1099/ijsem.0.001724
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发表时间：
2017
影响因子：
2.8
通讯作者：
Zhang Xiao-Xia
中科院分区：
文献类型：
--
作者：
Zhao Juan-Juan;Zhang Jun;Sun Lei;Zhang Rui-Jie;Zhang Cai-Wen;Yin Hua-Qun;Zhang Xiao-Xia
在水稻内生细菌多样性研究中，从鲜食水稻根部分离到2株革兰氏阴性、需氧、杆状内生细菌N19T和N11-2。16S rRNA基因序列测定结果表明，菌株N19T与N11-2的16S rRNA基因序列相似性为100 %。它们都属于根瘤菌属，与太白山根瘤菌CCNWSX 0483T的同源性为97.7 %，其次是根瘤菌NCPPB 3554T的同源性为97.5 %。这些新菌株与已建立的根瘤菌属成员之间看家基因recA、gyrBandglnA的序列相似性小于87 %。用初始复性速率法检测，菌株N19T与N11-2的杂交率为87.9 %。根据基因组草图序列，菌株N19T的TOR值分别为18.2dna%和19.6dna% %。太白山CCNWSX 0483TandR.Vitis S4，证明这些新菌株代表了根瘤菌属中的一个新种。主要的细胞脂肪酸总结为特征8(C18 ： 1ω7c和/或C18 ： 1ω6c)。菌株N19T的DNAG+C含量为58.7 摩尔%(TM)。N19T的极性脂谱由磷脂酰甘油、二磷脂酰甘油、磷脂酰乙醇胺、磷脂酰胆碱、一种未知脂、两种未知氨基脂和一种未知氨基磷脂组成。根据生理生化特性和基因分型数据，认为菌株N19T和N11-2是根瘤菌属的一个新种，命名为水稻根瘤菌(Rhizobium OryziRadissp)。11月N19T(=ACCC 19962T=KCTC 52413T)为模式菌株。
Two Gram-stain-negative, aerobic, rod-shaped endophytic bacterial strains, N19Tand N11-2, were isolated from fresh rice (Oryza sativa) roots during investigation of the rice endophytic bacterial diversity. The 16S rRNA gene sequence results indicated that the similarity between strains N19Tand N11-2 was 100 %. Both of them belong to the genusRhizobium, with close similarity toRhizobium taibaishanenseCCNWSX 0483T(97.7 %), followed byRhizobium vitisNCPPB 3554T(97.5 %). The sequence similarities of the housekeeping genesrecA,gyrBandglnAbetween the novel isolates and members of the established species of the genusRhizobiumwere less than 87 %. The DNA–DNA hybridization rates between strains N19Tand N11-2 were 87.9 % using the initial renaturation rate method. Based on draft genome sequences, strain N19Tshowed 18.2 % and 19.6 % DNA–DNA hybridization values toR. taibaishanenseCCNWSX 0483TandR. vitisS4, which demonstrated that these new isolates represent a novel species in the genusRhizobium. The main cellular fatty acids were summed feature 8 (C18 : 1ω7cand/or C18 : 1ω6c). The DNA G+C content of strain N19Twas 58.7 mol% (Tm). The polar lipid profile of N19Tconsisted of phosphatidylglycerol, diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylcholine, an unknown lipid, two unknown aminolipids and an unidentified aminophospholipid. According to physiological and biochemical characteristics and genotypic data, strains N19Tand N11-2 are considered to represent a novel species of the genusRhizobium, for which the nameRhizobium oryziradicissp. nov. is proposed, with N19T(=ACCC 19962T=KCTC 52413T) as the type strain.