Suppressors of glp-1, a gene required for cell communication during development in Caenorhabditis elegans, define a set of interacting genes.
Suppressors of glp-1, a gene required for cell communication during development in Caenorhabditis elegans, define a set of interacting genes.
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glp-1（秀丽隐杆线虫发育过程中细胞通讯所需的基因）的抑制因子定义了一组相互作用的基因。
DOI：
10.1093/genetics/135.4.1011
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发表时间：
1993
期刊：
影响因子：
3.3
通讯作者：
Kimble,J
中科院分区：
文献类型：
--
作者：
Maine,EM;Kimble,J
GLP-1基因是必不可少的两个细胞的相互作用，控制细胞的命运在秀丽隐杆线虫：诱导前咽在胚胎和诱导生殖细胞系中的有丝分裂增殖。为了鉴定参与这些细胞相互作用的其他基因，我们分离了glp-1的两个温度敏感等位基因的抑制子。14个隐性抑制基因中的每一个都能挽救胚胎和生殖系glp-1（ts）缺陷。这些抑制因子是基因外的，定义了一组六个基因，命名为sog，即glp-1的抑制因子。glp-1的抑制是与sog突变相关的唯一明显的表型。不同sog基因的突变显示等位基因特异性的基因间非互补，表明sog基因产物可能相互作用。此外，我们还分析了一个半显性突变，该突变仅抑制glp-1种系表型，并具有其自身的条件性女性化表型。没有一个抑制因子能挽救glp-1无效突变，因此它们不能绕过对glp-1的需求。远端细胞功能仍然是抑制动物生殖细胞增殖所必需的。这些抑制基因突变鉴定出可能编码glp-1介导的细胞信号传导途径的其他组分或调节glp-1表达的基因。
The glp-1 gene is essential for two cell interactions that control cell fate in Caenorhabditis elegans: induction of anterior pharynx in the embryo and induction of mitotic proliferation in the germ line. To identify other genes involved in these cell interactions, we have isolated suppressors of two temperature sensitive alleles of glp-1. Each of 14 recessive suppressors rescues both embryonic and germline glp-1(ts) defects. These suppressors are extragenic and define a set of six genes designated sog, for suppressor of glp-1. Suppression of glp-1 is the only obvious phenotype associated with sog mutations. Mutations in different sog genes show allele-specific intergenic noncomplementation, suggesting that the sog gene products may interact. In addition, we have analyzed a semidominant mutation that suppresses only the glp-1 germline phenotype and has a conditional feminized phenotype of its own. None of the suppressors rescues a glp-1 null mutation and therefore they do not bypass a requirement for glp-1. Distal tip cell function remains necessary for germline proliferation in suppressed animals. These suppressor mutations identify genes that may encode other components of the glp-1 mediated cell-signaling pathway or regulate glp-1 expression.