Tumour Expression of Histone Deacetylases in Uveal Melanoma
Tumour Expression of Histone Deacetylases in Uveal Melanoma
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DOI：
10.1159/000490038
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发表时间：
2019-01-01
影响因子：
1
通讯作者：
Cherepanoff, Svetlana
中科院分区：
文献类型：
--
作者：
Levinzon, Louis;Madigan, Michele;Cherepanoff, Svetlana
目的：研究组蛋白脱乙酰酶在葡萄膜黑色素瘤细胞中的表达。方法：这是2001年1月至2002年3月间因葡萄膜黑色素瘤摘除的16只福尔马林固定的石蜡包埋眼的观察性免疫组织化学研究。根据美国癌症联合委员会第7版，对苏木精和曙红石蜡切片的组织病理学参数进行了综述。然后使用自动Leica Bond II平台和Fast Red Chromogen对切片进行组蛋白脱乙酰酶1、2、3、4和6以及sirtuin 2的免疫组织化学染色，然后使用Aperio软件进行数字化扫描，然后进行染色评估。结果：葡萄膜黑色素瘤细胞有组蛋白脱乙酰酶1、2、3、4、6和sirtuin 2的核表达。此外，肿瘤细胞可见组蛋白脱乙酰酶4、6和sirtuin 2的胞浆表达。未受肿瘤侵袭的眼内组织也可见胞核和胞浆免疫染色。结论：葡萄膜黑色素瘤细胞表达组蛋白脱乙酰酶1、2、3、4、6和sirtuin 2，证实了组蛋白脱乙酰酶抑制剂的潜在组织靶点。(C)2018年S.Karger AG，巴塞尔
Purpose: To determine the expression of histone deacetylase enzymes in uveal melanoma tumour cells. Procedures: This is an observational immunohistochemical study of 16 formalin-fixed, paraffin-embedded eyes enucleated for uveal melanoma between January 2001 and March 2002. Haematoxylin and eosin paraffin sections were reviewed for histopathological parameters according to the American Joint Committee on Cancer 7th edition. Sections were then immunohistochemically stained for histone deacetylases 1, 2, 3, 4 and 6 and sirtuin 2 using an automated Leica Bond II platform and Fast Red chromogen, then digitally scanned using Aperio software before assessment of staining. Results: Nuclear expression of histone deacetylases 1, 2, 3, 4 and 6 and of sirtuin 2 was confirmed in uveal melanoma tumour cells. In addition, the tumour cells showed cytoplasmic expression of histone deacetylases 4 and 6 and sirtuin 2. Nuclear and cytoplasmic immunostaining was also seen in intraocular tissues uninvolved by the tumour. Conclusion: Uveal melanoma tumour cells express histone deacetylases 1, 2, 3, 4 and 6 and sirtuin 2, confirming potential tissue targets for histone deacetylase inhibitors. (C) 2018 S. Karger AG, Basel