New serotype of Bartonella henselae in endocarditis and cat-scratch disease
New serotype of Bartonella henselae in endocarditis and cat-scratch disease
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DOI：
10.1016/s0140-6736(96)90012-4
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发表时间：
1996-02-17
期刊：
影响因子：
168.9
通讯作者：
Raoult, D
中科院分区：
文献类型：
--
作者：
Drancourt, M;Birtles, R;Raoult, D
背景 汉赛巴尔通体的挑剔性使得临床感染的证明主要依赖于血清学或分子生物学方法。仅从猫抓病 (CSD) 患者中分离出 5 个分离株，而没有从心内膜炎患者中分离到。方法 我们从 CSD 患者中分离出汉赛双歧杆菌，这也是首次从心内膜炎患者中分离出。根据形态学、生物化学、细胞壁脂肪酸分析、16-23S 基因内间隔区的 PCR 限制性片段长度多态性分析和 16S rRNA 基因序列对分离株进行了表征。这些分离株的特征表明它们属于一个新的血清群，我们称之为“马赛”，并且属于基于 16S rRNA 基因序列的新基因型。新的变体被纳入免疫荧光抗体测试（IFAT）中，该测试用于重新评估 113 名传统 IFAT 血清阴性的 CSD 患者的血清样本。结果发现，这些血清阴性患者中有 18 名（16%）滴度显着升高。 20 名按传统 IFAT 判断为血清阳性的 CSD 患者在新 IFAT 中仍呈血清阳性。 解释 物种内的抗原变异性是 CSD 血清学诊断结果不一致的可能原因之一。
Background The fastidious nature of Bartonella henselae is such that demonstration of clinical infection relies mainly on serological or molecular biological methods. Only five isolates have been obtained from patients with cat-scratch disease (CSD) and none from endocarditis.Methods We isolated B henselae from a CSD patient and, for the first time, from a patient with endocarditis. The isolates were characterised on the basis of morphology, biochemistry, cell-wall fatty-acid analysis, PCR-restriction fragment length polymorphism analysis of the 16-23S intragenic spacer region, and 16S rRNA gene sequences. Characterisation of these isolates indicated them to belong to a new serogroup, which we have called ''Marseille'', and to a new genotype based on the 16S rRNA gene sequence. The new variant was incorporated into an immunofluorescence antibody test (IFAT), which was used to reassess serum samples from 113 CSD patients who were seronegative with the conventional IFAT. Findings 18 (16%) of these apparently seronegative patients yielded significantly raised titres. 20 CSD patients who were seropositive as judged by the conventional IFAT remained seropositive with the new IFAT.Interpretation Antigenic variability within the species is one possible reason for inconsistent results in the serological diagnosis of CSD.