CLONING AND STABLE MAINTENANCE OF 300-KILOBASE-PAIR FRAGMENTS OF HUMAN DNA IN ESCHERICHIA-COLI USING AN F-FACTOR-BASED VECTOR
CLONING AND STABLE MAINTENANCE OF 300-KILOBASE-PAIR FRAGMENTS OF HUMAN DNA IN ESCHERICHIA-COLI USING AN F-FACTOR-BASED VECTOR
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DOI：
10.1073/pnas.89.18.8794
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发表时间：
1992-09-15
影响因子：
11.1
通讯作者：
SIMON, M
中科院分区：
文献类型：
--
作者：
SHIZUYA, H;BIRREN, B;SIMON, M
建立了一套用于复杂基因组作图和分析的细菌克隆系统。细菌人工染色体的BAC系统是以大肠杆菌及其单拷贝质粒F因子为基础的。它能够保持人类基因组DNA片段的300千碱基对。人类DNA的个体克隆似乎在宿主中保持了高度的结构稳定性，即使在100代连续生长之后也是如此。由于克隆效率高，克隆的DNA易于操作，插入的DNA保持稳定，BAC系统有助于构建更具代表性的复杂基因组DNA文库，并随后对复杂基因组结构进行快速分析。
A bacterial cloning system for mapping and analysis of complex genomes has been developed. The BAC system (for bacterial artificial chromosome) is based on Escherichia coli and its single-copy plasmid F factor. It is capable of maintaining human genomic DNA fragments of >300 kilobase pairs. Individual clones of human DNA appear to be maintained with a high degree of structural stability in the host, even after 100 generations of serial growth. Because of high cloning efficiency, easy manipulation of the cloned DNA, and stable maintenance of inserted DNA, the BAC system may facilitate construction of DNA libraries of complex genomes with fuller representation and subsequent rapid analysis of complex genomic structure.