Comprehensive Proteomics Analysis of Laticifer Latex Reveals New Insights into Ethylene Stimulation of Natural Rubber Production.
Comprehensive Proteomics Analysis of Laticifer Latex Reveals New Insights into Ethylene Stimulation of Natural Rubber Production.
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DOI：
10.1038/srep13778
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发表时间：
2015-09-08
影响因子：
4.6
通讯作者：
Tong Z
中科院分区：
文献类型：
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作者：
Wang X;Wang D;Sun Y;Yang Q;Chang L;Wang L;Meng X;Huang Q;Jin X;Tong Z
乙烯是增加天然胶乳的刺激物。施用乙烯后，胶乳鲜重和干物质都有明显提高。此外，我们发现乙烯改善了小橡胶颗粒的产生。然而，大多数参与橡胶生物合成的基因被外源乙烯抑制。因此，我们进行了蛋白质组学分析的乙烯刺激的橡胶胶乳，并确定了287丰富的蛋白质，以及143乙烯响应乳胶蛋白（ERLPs）与质谱从2-DE和DIGE凝胶，分别。此外，iTRAQ还鉴定了1,600多种蛋白质，包括404种ERLP。ERLPs的功能分类显示，参与翻译后修饰、碳水化合物代谢、水解酶活性和激酶活性的酶过多。通过抑制胶粒聚集酶的作用，延长胶乳的流动时间，从而提高胶乳产量。磷酸化蛋白质组学分析确定了59个差异磷蛋白，特别是，橡胶延伸因子和小橡胶颗粒蛋白的磷酸化主要在丝氨酸残基的特定亚型。这种翻译后修饰和异构体特异性磷酸化可能是重要的乙烯刺激的胶乳生产。这些结果不仅加深了我们对橡胶胶乳蛋白质组的理解，而且为利用乙烯刺激橡胶胶乳生产提供了新的见解。
Ethylene is a stimulant to increase natural rubber latex. After ethylene application, both fresh yield and dry matter of latex are substantially improved. Moreover, we found that ethylene improves the generation of small rubber particles. However, most genes involved in rubber biosynthesis are inhibited by exogenous ethylene. Therefore, we conducted a proteomics analysis of ethylene-stimulated rubber latex, and identified 287 abundant proteins as well as 143 ethylene responsive latex proteins (ERLPs) with mass spectrometry from the 2-DE and DIGE gels, respectively. In addition, more than 1,600 proteins, including 404 ERLPs, were identified by iTRAQ. Functional classification of ERLPs revealed that enzymes involved in post-translational modification, carbohydrate metabolism, hydrolase activity, and kinase activity were overrepresented. Some enzymes for rubber particle aggregation were inhibited to prolong latex flow, and thus finally improved latex production. Phosphoproteomics analysis identified 59 differential phosphoproteins; notably, specific isoforms of rubber elongation factor and small rubber particle protein that were phosphorylated mainly at serine residues. This post-translational modification and isoform-specific phosphorylation might be important for ethylene-stimulated latex production. These results not only deepen our understanding of the rubber latex proteome but also provide new insights into the use of ethylene to stimulate rubber latex production.