Vertical and horizontal gene transfer tradeoffs direct plasmid fitness.
Vertical and horizontal gene transfer tradeoffs direct plasmid fitness.
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垂直和水平基因转移权衡直接质粒适应性。
DOI：
10.15252/msb.202211300
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发表时间：
2023-02-10
影响因子：
9.9
通讯作者：
中科院分区：
文献类型：
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作者：
质粒适应性由两个正交过程控制——通过细胞分裂的垂直转移和通过接合的水平转移。单独考虑时，任何一种转移模式的改进都可以促进质粒传播和持续的良好程度。然而，结合的代谢成本可能会产生限制质粒进化的权衡。在这里，我们提供了来自临床大肠杆菌病原体的 40 个质粒中接合生长权衡的存在、后果和分子基础的证据。我们发现大多数质粒在主要生长效应的接合效率阈值以下运行，表明垂直转移的强烈自然选择。低于此阈值，大肠杆菌对接合效率超过四个数量级的变化表现出显着的生长耐受性。随着营养物质变得稀缺并且水平转移吸引了更大份额的宿主资源，这种耐受性逐渐消失。我们的结果提供了对指导质粒适应性的进化限制和对抗抗生素耐药性传播的策略的见解。自然质粒转移率的量化揭示了限制质粒进化的接合和细胞分裂之间的权衡。
Plasmid fitness is directed by two orthogonal processes—vertical transfer through cell division and horizontal transfer through conjugation. When considered individually, improvements in either mode of transfer can promote how well a plasmid spreads and persists. Together, however, the metabolic cost of conjugation could create a tradeoff that constrains plasmid evolution. Here, we present evidence for the presence, consequences, and molecular basis of a conjugation‐growth tradeoff across 40 plasmids derived from clinical Escherichia coli pathogens. We discover that most plasmids operate below a conjugation efficiency threshold for major growth effects, indicating strong natural selection for vertical transfer. Below this threshold, E. coli demonstrates a remarkable growth tolerance to over four orders of magnitude change in conjugation efficiency. This tolerance fades as nutrients become scarce and horizontal transfer attracts a greater share of host resources. Our results provide insight into evolutionary constraints directing plasmid fitness and strategies to combat the spread of antibiotic resistance. Quantification of natural plasmid transfer rates reveals a tradeoff between conjugation and cell division that constrains plasmid evolution.