PDMS device for patterned application of microfluids to neuronal cells arranged by microcontact printing
PDMS device for patterned application of microfluids to neuronal cells arranged by microcontact printing
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DOI：
10.1016/s0956-5663(01)00279-2
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发表时间：
2002-01-01
影响因子：
12.6
通讯作者：
Offenhäusser, A
中科院分区：
文献类型：
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作者：
Thiébaud, P;Lauer, L;Offenhäusser, A
在聚二甲基硅氧烷(PDMS)中实现了一种由基流通道中集成的八线微注入阵列组成的微流控装置。该装置由多个PDMS部件组装而成，这些部件是使用SU-8光刻胶的微机械母版模压而成的。在与平面衬底接触时，最多可以产生8条独立的层流流线，横截面为100 x 200微米(2)。该装置致力于将药物化合物应用于体外产生电的细胞，并用神经细胞系MZL-Cells进行了测试。这些细胞被培养在通过微接触印刷沉积到聚苯乙烯基质上的层粘连蛋白线路上。我们能够在细胞生长方向平行的情况下将多条有色PBS注入到该培养物中。单独的流线之间没有发生混合。(C)2002 Elsevier Science B.V.保留所有权利。
A microfluidic device in polydimethylsiloxane (PDMS) consisting of an eight lines micro-injection array integrated in a base flow channel has been realized. The device is assembled from multiple PDMS parts, which have been moulded using notably micromachined masters in SU-8 photoresist. In contact with a planar substrate., up to eight independent laminar flow lines with cross-sections of 100 x 200 mum(2) can be generated. Dedicated for the application of pharmaceutical compounds to electrogenic cells in vitro, this device was tested with a neuronal cell line, Mzl-cells. These were cultured on lines of laminin deposited onto polystyrene substrates by microcontact printing. We were able to inject into this culture multiple lines of coloured PBS in parallel to the orientation of cellular growth. No mixing between the individual flow lines did occur. (C) 2002 Elsevier Science B.V. All rights reserved.