The use of high-performance liquid chromatography for quantitation of plasma amino acids in man.
The use of high-performance liquid chromatography for quantitation of plasma amino acids in man.
复制标题
使用高效液相色谱法定量人体血浆氨基酸。
DOI：
10.1016/0003-2697(82)90138-5
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发表时间：
1982
影响因子：
2.9
通讯作者：
Isenberg,JI
中科院分区：
文献类型：
--
作者：
Hogan,DL;Kraemer,KL;Isenberg,JI
初级氨基酸与邻苯二甲醛的柱前衍生化生成荧光衍生物，可通过反相高效液相色谱(HPLC)分离。人血浆经脱蛋白和衍生化后，在不到35min的时间内产生单一的氨基酸峰。微量血浆游离氨基酸的定量分析具有很高的重复性(平均变异系数2.9±0.5%；r=0.988±0.005；p<；0.001)。用高效液相色谱法与Beckman119型和120B型氨基酸分析仪测定血浆氨基酸具有良好的一致性，变异系数分别为13.4±4.3%和19.0±3.6%，r分别为0.936和0.711。蛋白质餐后血浆氨基酸显著增加。高效液相色谱法是一种快速、灵敏、准确的测定人体血浆氨基酸的方法。
Precolumn derivatization of primary amino acids with o-phthaldialdehyde forms fluorescent derivatives that can be separated by reverse-phase high-performance liquid chromatography (HPLC). Human plasma deproteinized and then derivatized by this method produced individual amino acid peaks in less than 35 min. Quantitative analysis of micro amounts of plasma-free amino acids gave highly reproducible results (mean coefficient of variation 2.9 ± 0.5%; r = 0.988 ± 0.005; p < 0.001). There was good agreement between measurement of plasma amino acids with HPLC and Beckman 119 and 120B amino acid analyzers; coefficient of variation 13.4 ± 4.3% and 19.0 ± 3.6%, respectively; r = 0.936 and 0.711, respectively (p < 0.05). There was a significant increase in plasma amino acids after a protein meal. HPLC is a rapid, sensitive, and precise method of measuring plasma amino acids in man.