THE EFFECT OF DMSO ON SEVERAL ENZYME SYSTEMS *
THE EFFECT OF DMSO ON SEVERAL ENZYME SYSTEMS *
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DMSO 对几种酶系统的影响*
DOI：
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发表时间：
1967
影响因子：
5.2
通讯作者：
D. Rammler
中科院分区：
文献类型：
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作者：
D. Rammler
蛋白质二级和三级分子结构的稳定性取决于其溶剂环境的性质，以及温度、离子强度和pH等因素（Schachman，1963; Straub，1964）。在医学中，二甲亚砜（DMSO）通常在体温下以相对高的浓度使用（Jacob等人，1964年; Kligman，1965），预期会改变蛋白质构象的条件（Hamaguchi，1964;参见Rammler & Zaffaroni以总结参考文献）。通常，通过测量蛋白质的一些物理性质的差异来确定构象变化（Hermans，1965）;然而，溶剂诱导的酶活性变化也是蛋白质构象变化的有用指标，因为酶的活性通常受到蛋白质结构变化的极大影响（Singer，1962; Monod等人，1963年）。在这份初步报告中，我们希望提出我们的调查结果有关的影响，DMSO对几个酶系统和恢复这些系统后，除去DMSO。
The stability of the secondary and tertiary molecular structure of a protein is dependent upon the nature of its solvent environment, in addition to such factors as temperature, ionic strength and pH (Schachman, 1963; Straub, 1964). In medicine, dimethyl sulfoxide (DMSO) is generally used a t body temperature and in relatively high concentrations (Jacob et al., 1964; Kligman, 1965), conditions that would be expected to alter the conformation of proteins (Hamaguchi, 1964; see Rammler & Zaffaroni for summarizing references). In general, conformational changes are determined by measuring a difference in some physical property of the protein (Hermans, 1965); however, solvent-induced changes in enzymatic activity are also useful indicators of protein conformational changes, because an enzyme’s activity is generally greatly affected by changes in protein structure (Singer, 1962; Monod et al., 1963). In this preliminary report, we wish to present results of our investigations concerned with the effect of DMSO on several enzymatic systems and the recovery of these systems after removal of the DMSO.