Nitro-oleic acid triggers ROS production via NADPH oxidase activation in plants: A pharmacological approach
Nitro-oleic acid triggers ROS production via NADPH oxidase activation in plants: A pharmacological approach
复制标题
硝基油酸通过激活NADPH氧化酶激活植物体内ROS的药理学研究
DOI：
10.1016/j.jplph.2020.153128
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发表时间：
2020-03-01
影响因子：
4.3
通讯作者：
Laxalt, Ana M.
中科院分区：
文献类型：
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作者：
Di Palma, Andres Arruebarrena;Di Fino, Luciano M.;Laxalt, Ana M.
硝化脂肪酸(NO2-FA)是哺乳动物体内重要的信号分子。NO2-FAs是由一氧化氮和亚硝酸盐衍生的二氧化氮与不饱和脂肪酸双键加成反应形成的。植物系统中NO2-FAs的研究是一个有趣的新兴领域。据报道，在橄榄、豌豆、水稻和拟南芥中都存在NO2-FA。为了更好地了解NO2-FA在植物生理中的作用，我们分析了外源施用硝基油酸(NO2-OA)的效应。在番茄细胞悬浮液中，我们发现NO2-OA通过激活NADPH氧化酶以剂量依赖的方式诱导活性氧(ROS)的产生，这一机制需要细胞外钙进入和蛋白激酶激活。在番茄和拟南芥叶片中，NO2-OA处理诱导了两波ROS的产生，类似于植物的防御反应。拟南芥NADPH氧化酶突变体表明，NADPH异构体D(RBOHD)是NO2-OA诱导ROS产生所必需的。此外，在拟南芥的离体表皮上，NO2-OA通过RBOHD和F.诱导气孔关闭。这些结果表明，NO2-OA激活了NADPH氧化酶，揭示了植物中一种新的信号作用。
Nitrated fatty acids (NO2-FAs) are important signaling molecules in mammals. NO2-FAs are formed by the addition reaction of nitric oxide- and nitrite-derived nitrogen dioxide with unsaturated fatty acid double bonds. The study of NO2-FAs in plant systems constitutes an interesting and emerging area. The presence of NO2-FA has been reported in olives, peas, rice and Arabidopsis. To gain a better understanding of the role of NO2-FA on plant physiology, we analyzed the effects of exogenous application of nitro-oleic acid (NO2-OA). In tomato cell suspensions we found that NO2-OA induced reactive oxygen species (ROS) production in a dose-dependent manner via activation of NADPH oxidases, a mechanism that requires calcium entry from the extracellular compartment and protein kinase activation. In tomato and Arabidopsis leaves, NO2-OA treatments induced two waves of ROS production, resembling plant defense responses. Arabidopsis NADPH oxidase mutants showed that NADPH isoform D (RBOHD) was required for NO2-OA-induced ROS production. In addition, on Arabidopsis isolated epidermis, NO2-OA induced stomatal closure via RBOHD and F. Altogether, these results indicate that NO2-OA triggers NADPH oxidase activation revealing a new signaling role in plants.