Determining DNA packaging strategy by analysis of the termini of the chromosomes in tailed-bacteriophage virions.
Determining DNA packaging strategy by analysis of the termini of the chromosomes in tailed-bacteriophage virions.
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DOI：
10.1007/978-1-60327-565-1_7
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发表时间：
2009
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影响因子：
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通讯作者：
Gilcrease, Eddie B
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作者：
Casjens, Sherwood R;Gilcrease, Eddie B
有尾噬菌体病毒体含有单个线性dsDNA染色体，其在已知的有尾噬菌体类型中的大小范围可以为约18至500 kbp。这些线性染色体可以具有以下几种已知类型的末端之一：粘性末端（5′-或3′-单链延伸），环状排列的直接末端重复，短或长的精确直接末端重复，末端宿主DNA序列或共价结合的末端蛋白质。这些不同类型的末端反映了不同的DNA复制策略，特别是在DNA包装过程中不同的末端酶作用。一般来说，完整的基因组序列测定本身并不能阐明这些末端的性质，因此通常需要定向实验分析来了解病毒体染色体末端的性质。本章讨论这些方法。
Tailed-bacteriophage virions contain a single linear dsDNA chromosome which can range in size from about 18 to 500 kbp across the known tailed-phage types. These linear chromosomes can have one of several known types of termini as follows: cohesive ends (5′- or 3′-single-strand extensions), circularly permuted direct terminal repeats, short or long exact direct terminal repeats, terminal host DNA sequences, or covalently bound terminal proteins. These different types of ends reflect differing DNA replication strategies and especially differing terminase actions during DNA packaging. In general, complete genome sequence determination does not by itself elucidate the nature of these ends, so directed experimental analysis is usually required to understand the nature of the virion chromosome ends. This chapter discusses these methods.