Visualizing spatiotemporal dynamics of multicellular cell-cycle progression
Visualizing spatiotemporal dynamics of multicellular cell-cycle progression
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DOI：
10.1016/j.cell.2007.12.033
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发表时间：
2008-02-08
期刊：
影响因子：
64.5
通讯作者：
Miyawaki, Atsushi
中科院分区：
文献类型：
--
作者：
Sakaue-Sawano, Asako;Kurokawa, Hiroshi;Miyawaki, Atsushi
细胞周期从G（1）期到S期的转变一直难以可视化。我们已经利用了标记细胞周期转换的反相振荡蛋白，以开发用于此目的的遗传编码荧光探针。这些探针有效地将单个G 1相核标记为红色，将S/G（2）/M相核标记为绿色。我们能够产生培养的细胞和组成型表达细胞周期探针的转基因小鼠，其中每个细胞核显示红色或绿色荧光。我们进行了延时成像，以探索培养细胞的上皮-间充质转化过程中细胞周期动力学的时空模式，脑切片中神经祖细胞的迁移和分化，以及活体小鼠血管中肿瘤的发展。这些小鼠和细胞系将作为模型系统，允许前所未有的空间和时间分辨率，以帮助我们更好地了解细胞周期如何与各种生物事件协调。
The cell-cycle transition from G(1) to S phase has been difficult to visualize. We have harnessed antiphase oscillating proteins that mark cell-cycle transitions in order to develop genetically encoded fluorescent probes for this purpose. These probes effectively label individual G, phase nuclei red and those in S/G(2)/M phases green. We were able to generate cultured cells and transgenic mice constitutively expressing the cell-cycle probes, in which every cell nucleus exhibits either red or green fluorescence. We performed time-lapse imaging to explore the spatiotemporal patterns of cell-cycle dynamics during the epithelial-mesenchymal transition of cultured cells, the migration and differentiation of neural progenitors in brain slices, and the development of tumors across blood vessels in live mice. These mice and cell lines will serve as model systems permitting unprecedented spatial and temporal resolution to help us better understand how the cell cycle is coordinated with various biological events.