Preparation and characterization of chitosan/galactosylated hyaluronic acid scaffolds for primary hepatocytes culture
Preparation and characterization of chitosan/galactosylated hyaluronic acid scaffolds for primary hepatocytes culture
复制标题
原代肝细胞培养用壳聚糖/半乳糖化透明质酸支架的制备及表征
DOI：
10.1007/s10856-009-3833-y
复制
发表时间：
2010-01-01
影响因子：
3.7
通讯作者：
Yang, Jun
中科院分区：
文献类型：
--
作者：
Fan, Jinyong;Shang, Yi;Yang, Jun
肝细胞特异性三维组织工程支架在开发生物人工肝装置中发挥着重要作用。在本研究中，半乳糖部分通过乙二胺与透明质酸共价偶联。采用冷冻干燥技术制备了壳聚糖(CS)和半乳糖化透明质酸(GHA)复合海绵。用扫描电子显微镜观察了支架的形貌。GHA含量和冻结温度对海绵的孔隙率和孔径有很大的影响。GHA的加入不仅改善了材料的润湿性，改变了材料的力学性能，而且显著影响了材料的细胞贴壁率。此外，CS/GHA支架与壳聚糖支架相比，肝细胞的白蛋白分泌、尿素合成和氨排出等肝功能均得到改善。
Hepatocyte-specific three-dimensional tissue-engineeringed scaffold plays an important role for developing bioartificial liver devices. In the present study, galactose moieties were covalently coupled with hyaluronic acid through ethylenediamine. Highly porous sponge composed of chitosan (CS) and galactosylated hyaluronic acid (GHA) was prepared by freezing-drying technique. The morphology of the scaffolds was observed via scanning electron microscopy. Porosity and pore size of the sponge were greatly dependent on the content of GHA and freezing temperature. The addition of GHA not only improved the wettability and changed their mechanical properties, but also significantly influenced the cell attachment ratio. Moreover, liver functions of the hepatocytes such as albumin secretion, urea synthesis and ammonia elimination in the CS/GHA scaffolds were improved in comparison with those in the chitosan scaffolds.