Sexual dimorphism in the effects of mating on the in vitro release of LHRH from the ferret mediobasal hypothalamus.
Sexual dimorphism in the effects of mating on the in vitro release of LHRH from the ferret mediobasal hypothalamus.
复制标题
交配对雪貂下丘脑中基底层体外 LHRH 释放影响的性别二态性。
DOI：
10.1016/0031-9384(92)90418-2
复制
发表时间：
1992
影响因子：
2.9
通讯作者：
Baum,MJ
中科院分区：
文献类型：
--
作者：
Lambert,GM;Rubin,BS;Baum,MJ
黄体生成素（LH）分泌的两性二态模式先前已被证明发生在诱导排卵物种雪貂的交配反应中，交配增加了雌性黄体生成素（LH）的分泌，而雄性则没有。本研究的目的是确定性交后黄体生成素分泌的这种二态模式是否反映了交配对黄体生成素释放激素（LHRH）神经释放的二态效应。研究了交配对繁殖雄性和雌性雪貂中基底下丘脑（MBH） LHRH体外释放的影响。交配后0.25 h牺牲的发情雌鼠与未配对的发情雌鼠和交配刺激后1 h或2.6 h牺牲的发情雌鼠相比，组织中黄体生成素的释放量和含量显著减少。相比之下，在交配后0.25 h牺牲的繁殖雄性和未配对的繁殖雄性中，从MBH片段中释放的LHRH和LHRH组织含量相当。这些数据表明，雌性雪貂性交后黄体生成素的激增是由LHRH的释放引起的，LHRH的释放最初会消耗MBH内的神经末梢，而雄性雪貂的LHRH释放与垂体黄体生成素分泌一样，受交配的影响最小。
A sexually dimorphic pattern in the secretion of luteinizing hormone (LH) has previously been shown to occur in response to mating in an induced ovulating species, the ferret, with mating augmenting the secretion of LH in females but not in males. The aim of this study was to determine whether this dimorphic pattern in the postcoital secretion of LH reflects a dimorphic effect of mating on the neural release of luteinizing hormone releasing hormone (LHRH). The effect of mating on the in vitro release of LHRH from mediobasal hypothalami (MBH) collected from breeding male and female ferrets was studied. Luteinizing hormone releasing hormone release and content were significantly reduced in tissues from estrous females sacrificed 0.25 h after mating compared to unpaired estrous females and estrous females sacrificed 1 or 2.6 h after the mating stimulus. By contrast, the release of LHRH from MBH fragments and LHRH tissue content were equivalent in breeding males that were sacrificed 0.25 h after mating and in breeding males that were left unpaired. These data suggest that the postcoital surge of LH in the female ferret is preceded by a release of LHRH that initially depletes neuronal terminals within the MBH, whereas LHRH release, like pituitary LH secretion, is minimally affected by mating in males.