PREVALENCE OF EPSTEIN-BARR-VIRUS AND HUMAN PAPILLOMAVIRUS IN ORAL-MUCOSA OF HIV-INFECTED PATIENTS
PREVALENCE OF EPSTEIN-BARR-VIRUS AND HUMAN PAPILLOMAVIRUS IN ORAL-MUCOSA OF HIV-INFECTED PATIENTS
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DOI：
10.1111/j.1600-0714.1992.tb00095.x
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发表时间：
1992-04-01
影响因子：
3.3
通讯作者：
SHILLITOE, EJ
中科院分区：
文献类型：
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作者：
ADLERSTORTHZ, K;FICARRA, G;SHILLITOE, EJ
EB病毒(EBV)与口腔毛状白斑(OHL)的发生有关。最初，OHL也与人乳头状瘤病毒(HPV)有关，其证据是用抗人乳头瘤病毒(HPV)共同结构抗原的抗血清染色，以及原位DNA杂交检测到两例HPV阳性的OHL。本研究的目的是确定EBV和HPVDNA在OHL和正常口腔黏膜中的流行率，并解释OHL组织中抗乳头瘤病毒共同结构抗原抗体染色的基础。用原位杂交法检测了47例人类免疫缺陷病毒(HIV)阳性OHL中的47例和10例临床正常口腔黏膜组织中的1例EBV DNA。35例OHL标本中有25例乳头瘤病毒共同结构抗原抗体染色阳性。两株含EBV的淋巴母细胞株均未见染色，表明与抗乳头瘤病毒抗体的染色不是由于与EBV抗原的抗原交叉反应所致。聚合酶链式反应扩增法检测18例OHL中10例和10例正常口腔黏膜中6例HPVDNA。我们的结果表明，EBV和HPV在OHL中频繁存在，HPV在组织学正常的粘膜中也经常存在。
Epstein-Barr virus (EBV) has been implicated in the genesis of oral hairy leukoplakia (OHL). Initially, OHL was also associated with human papillomavirus (HPV) as evidenced by staining with antiserum to papillomavirus common structural antigens and reports of two HPV-positive OHL as detected by in situ DNA hybridization. The aims of this study were to determine the prevalence of EBV and HPV DNA in OHL and normal oral mucosa and to explain the basis for the staining of OHL tissues with antibodies to papillomavirus common structural antigens. EBV DNA was detected by in situ hybridization in 47 of 47 cases of OHL from human immunodeficiency virus (HIV)-seropositive individuals and in 1 of 10 biopsies of clinically normal buccal mucosa from the same group of individuals. Twenty-five of 35 OHL specimens stained with antibody to papillomavirus common structural antigens. There was no staining of two EBV-containing lymphoblastoid lines, indicating that the staining with anti-papillomavirus antibody was not due to antigenic cross-reactivity with EBV antigens. HPV DNA was detected by polymerase chain reaction amplification in 10 of 18 OHL specimens and in 6 of 10 normal buccal mucosa specimens. Our results indicate that EBV and HPV are present frequently in OHL and that HPV can be found regularly in histologically normal mucosa.