Dynamic ray tracing for modeling optical cell manipulation.
Dynamic ray tracing for modeling optical cell manipulation.
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DOI：
10.1364/oe.18.016702
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发表时间：
2010-08-02
期刊：
影响因子：
3.8
通讯作者：
Eggleton CD
中科院分区：
文献类型：
--
作者：
Sraj I;Szatmary AC;Marr DW;Eggleton CD
当前用于预测由于光学捕获力而导致的细胞表面应力分布的方法基于固定几何形状的传统射线光学方案。细胞通常被建模为实心球，因为这有利于光力计算。然而，在这种作用力下，真实的和非刚性的细胞可能会变形，因此传统射线光学方法中固有的假设开始失效。在这项工作中，我们实现了动态光线追踪技术来计算由光捕获引起的可变形单元上的应力分布。在这里，细胞被建模为具有离散表面的三维弹性胶囊，并使用浸入边界法计算出相关的流体动力。我们使用这种方法来模拟球形、椭圆形和双凹胶囊由于单个二极管棒光阱在一定光功率范围内引起的外部光学力而产生的瞬态变形。
Current methods for predicting stress distribution on a cell surface due to optical trapping forces are based on a traditional ray optics scheme for fixed geometries. Cells are typically modeled as solid spheres as this facilitates optical force calculation. Under such applied forces however, real and non-rigid cells can deform, so assumptions inherent in traditional ray optics methods begin to break down. In this work, we implement a dynamic ray tracing technique to calculate the stress distribution on a deformable cell induced by optical trapping. Here, cells are modeled as three-dimensional elastic capsules with a discretized surface with associated hydrodynamic forces calculated using the Immersed Boundary Method. We use this approach to simulate the transient deformation of spherical, ellipsoidal and biconcave capsules due to external optical forces induced by a single diode bar optical trap for a range of optical powers.