Retardation of cell growth by avian reovirus p17 through the activation of p53 pathway.
Retardation of cell growth by avian reovirus p17 through the activation of p53 pathway.
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通过p53途径的激活，通过禽肠疾病病毒p17对细胞生长的延迟。
DOI：
10.1016/j.bbrc.2005.08.149
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发表时间：
2005-10-21
影响因子：
3.1
通讯作者：
Shih WL
中科院分区：
文献类型：
--
作者：
Liu HJ;Lin PY;Lee JW;Hsu HY;Shih WL
禽呼肠孤病毒S1基因片段的第二个开放阅读框编码一个17 kDa的非结构蛋白，命名为p17。到目前为止，p17的生物学作用还完全不清楚。通过台盼蓝拒染法和四甲基偶氮唑盐比色法，我们证实了p17的异位表达导致Vero、BHK、293和HeLa细胞的活细胞数和细胞增殖率下降。LDH活性测定和DNA片段化分析表明，p17的表达不会导致细胞死亡或凋亡。这些数据表明，p17具有生长抑制功能。半定量RT-PCR和Western blotting结果显示，p17表达的细胞以时间和剂量依赖的方式诱导CDK抑制物p21cip1/waf1的表达，而CDK抑制物p15INK4b、p16INK4a和p27kip的转录没有改变。在p17存在的情况下，P53蛋白水平和P53驱动的报告活性显著升高。显性阴性P53可减轻p17诱导的p21积聚、P53激活和生长抑制作用。综上所述，这些研究揭示了p17可能通过激活p53和p21cip1/waf1而在生长调节中发挥内在功能。
The second open reading frame of avian reovirus S1 gene segment encodes a 17 kDa non-structural protein, named p17. The biological role of p17 is fully unknown so far. Using trypan blue dye exclusion and MTT assay, we demonstrated that the ectopic expression of p17 results in the reduction of viable cell number and cell proliferation rate of Vero, BHK, 293, and HeLa cells. Measurement of LDH activity and DNA fragmentation analysis revealed that p17 expression did not cause cell death or apoptosis. These data indicated that the p17 possessed the growth retardation function. Semi-quantitative RT-PCR and Western blotting revealed that p17-expressing cells induced the expression of CDK inhibitor p21cip1/waf1 in a time- and dose-dependent manner, but the transcripts of CDK inhibitor p15INK4b, p16INK4a, or p27kip were not altered. In the presence of p17, the p53 protein level and p53-driven reporter activity were elevated significantly. Dominant negative p53 alleviated the p21 accumulation, p53 activation, and growth inhibition effect induced by p17. Taken together, these studies revealed a possible intrinsic function of p17 in growth regulation through the activation of p53 and p21cip1/waf1.
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影响因子：
5.4
作者：
Costas, C;Martínez-Costas, J;Benavente, J
通讯作者：
Benavente, J
影响因子：
64.5
作者：
ELDEIRY, WS;TOKINO, T;VOGELSTEIN, B
通讯作者：
VOGELSTEIN, B
DOI：
10.20506/rst.19.2.1237
发表时间：
2000-08-01
影响因子：
0.6
作者：
Jones, RC
通讯作者：
Jones, RC
影响因子：
5.4
作者：
Hobbs, WE;DeLuca, NA
通讯作者：
DeLuca, NA
影响因子：
2.2
作者：
SZEKERES, J;PACSA, AS;PEJTSIK, B
通讯作者：
PEJTSIK, B